Department of Animal Production Techniques, Al-Musaib Technical College, Al-Furat Al-Awsat Technical University, Babylon, Iraq.
Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, 20-950, Lublin, Poland.
Poult Sci. 2022 Oct;101(10):102066. doi: 10.1016/j.psj.2022.102066. Epub 2022 Jul 26.
The aim of study was to evaluate microbial and hatchability traits as well as chicks quality after hatching eggs disinfection with aqueous solutions of ginger (GR), garlic (GC), oregano (O), and cinnamon (C) extracts. The experiment was divided into 2 stages, at preliminary in vitro stage antimicrobial susceptibility of plant extracts (PEs) was tested against reference strains from the American Type Culture Collection. O and GC extracts had the best antimicrobial properties (P < 0.05). Then in in vivo stage 2,400 Japanese quails hatching eggs were divided into 6 groups, 400 eggs each. Eggs from first group were not disinfected (NC, negative control), eggs from second group were disinfected by formalin fumigation (C, positive control), in other groups 5% aqueous solutions of plant extracts of GR, GC, O, C were applied by spraying respectively. After standard incubation fertility, hatchability and periodical embryonic mortality were calculated as well as the body weight and livability of chickens during 14 d of rearing. Egg disinfection by aqueous solution of PEs led to maintain the hatchability, chick weight at hatch and post hatch body weight and early mortality of birds. Exclusion of any fungal isolates on eggshell surface was induced by GC followed by O and C groups (P < 0.05). In case of the bacteria colonies reduction only GR extract was effective. Chosen plant extracts may be treated as safe and alternative substances to traditional disinfectants of hatching eggs.
本研究旨在评估消毒鸡蛋后微生物和孵化率特性以及雏鸡质量，方法是使用姜（GR）、大蒜（GC）、牛至（O）和肉桂（C）提取物的水溶液对种蛋进行消毒。该实验分为两个阶段，在初步的体外阶段，测试了植物提取物（PEs）对美国典型培养物保藏中心标准菌株的抗菌敏感性。O 和 GC 提取物具有最佳的抗菌特性（P < 0.05）。然后在体内阶段 2，将 2400 只日本鹌鹑孵化蛋分为 6 组，每组 400 个。第一组鸡蛋未经消毒（NC，阴性对照），第二组鸡蛋用福尔马林熏蒸消毒（C，阳性对照），其他组分别用 5%GR、GC、O 和 C 的植物提取物水溶液喷雾消毒。在标准孵化后，计算了受精率、孵化率和定期胚胎死亡率，以及饲养 14 天后雏鸡的体重和成活率。PEs 水溶液消毒可保持孵化率、孵化时雏鸡体重和孵化后体重以及早期死亡率。GC 组后 followed 有 O 和 C 组可排除蛋壳表面的任何真菌分离株（P < 0.05）。在减少细菌菌落方面，只有 GR 提取物有效。所选植物提取物可作为孵化鸡蛋的传统消毒剂的安全替代物。
