Institute of Pathogens and Vectors, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali University, Dali, Yunnan 671000, China.
School of Public Health, Dali University, Dali, Yunnan 671000, China.
Korean J Parasitol. 2022 Aug;60(4):273-279. doi: 10.3347/kjp.2022.60.4.273. Epub 2022 Aug 24.
Laelapinae mites are involved in transmission of microbial diseases between wildlife and humans, with an impact on public health. In this study, 5 mite members in the subfamily Laelapinae (laelapin mites; LM) were morphologically identified by light microscopy, and the phylogenetic relationship of LM was analyzed in combination with the sequence information of part of the LM cytochrome oxidase subunit I (cox1) gene. The morphological identification revealed that 5 mites belonged to the genera Laelaps and Haemolaelaps, respectively. Sequence analysis showed that the ratio of non-synonymous mutation rate to synonymous mutation rate of LM was less than 1, indicating that the LM cox1 gene had undergone purifying selection. Phylogenetic analysis showed that the Laelapinae is a monophyletic group. The genera Haemolaelaps and Hyperlaelaps did not separated into distinct clades but clustered together with species of the genus Laelaps. Our morphological and molecular analyses to describe the phylogenetic relationships among different genera and species of Laelapinae provide a reference for the improvement and revision of the LM taxonomy system.
嗜革螨亚科螨类在野生动物和人类之间传播微生物疾病方面发挥作用，对公共卫生有影响。本研究通过光学显微镜对嗜革螨亚科的 5 种螨类（嗜革螨；LM）进行了形态学鉴定，并结合部分 LM 细胞色素氧化酶亚基 I（cox1）基因序列信息分析了 LM 的系统发育关系。形态学鉴定表明，这 5 种螨分别属于嗜革螨属和血革螨属。序列分析显示，LM 的非同义突变率与同义突变率的比值小于 1，表明 LM cox1 基因经历了纯化选择。系统发育分析表明，嗜革螨亚科是一个单系群。血革螨属和超革螨属并未分化为明显的分支，而是与嗜革螨属的物种聚集在一起。我们对不同属和种的嗜革螨亚科进行的形态学和分子分析，为改进和修订 LM 分类系统提供了参考。
