Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors, Dali University, Dali, Yunnan 671000, China.
Parasitology. 2023 Jun;150(7):644-652. doi: 10.1017/S0031182023000367. Epub 2023 Apr 13.
is a gamasid mite that is of medical and veterinary significance as parasites and vectors of disease agents, which can carry pathogens of zoonosis such as , , , , and . At present, only single mitochondrial genes have been analysed for in the world, and no complete mitochondrial genome has been reported. However, information carried by a single gene is limited. Therefore, the complete mitochondrial genome of was determined for the first time by Illumina Hiseq X-Ten platform in this study. The mitochondrial genome is 15 736 bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region of 1561 bp in length. Codon analysis of 13 protein-coding genes revealed that UUU, UUA, AUU, AUA and AAU were the most frequently used, while had the fastest evolutionary rate and the slowest. Comparative analysis of genome structure and breakpoint distances of the mitochondrial genomes of 23 species in 17 genera from 10 families of Gamasida deposited in GenBank revealed a novel gene arrangement type of the mitochondrial genome, and different degrees of rearrangement among different taxa of Gamasida. Phylogenetic analyses of Gamasida were performed using the maximum likelihood and Bayesian inference methods. was clustered with Dermanyssoidea and formed a more supportive sister group with This study provides novel insights into rearrangement patterns and evolution of mitochondrial genomes of Gamasida.
是一种革螨，作为寄生虫和疾病媒介具有医学和兽医意义，可携带人畜共患病原体，如、、、、和。目前，世界上仅对进行了单一线粒体基因分析，尚未报道完整的线粒体基因组。然而，单个基因所携带的信息是有限的。因此，本研究首次通过 Illumina Hiseq X-Ten 平台确定了的完整线粒体基因组。线粒体基因组长 15736bp，包含 13 个蛋白质编码基因、22 个 tRNA 基因、2 个 rRNA 基因和长 1561bp 的控制区。对 13 个蛋白质编码基因的密码子分析表明，UUU、UUA、AUU、AUA 和 AAU 使用频率最高，而进化速度最快，最慢。对 GenBank 中 10 科 17 属 23 种革螨线粒体基因组的基因组结构和断点距离的比较分析表明，革螨线粒体基因组具有一种新的基因排列类型，不同分类群的革螨之间存在不同程度的重排。使用最大似然法和贝叶斯推断法对革螨进行了系统发育分析。与 Dermanyssoidea 聚类，并与形成更支持的姐妹群。本研究为革螨线粒体基因组的重排模式和进化提供了新的见解。
