Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors, Dali University, Dali, 671000, Yunnan, China.
Exp Appl Acarol. 2024 Oct;93(3):515-535. doi: 10.1007/s10493-024-00943-2. Epub 2024 Jul 17.
We collected 56 specimens of Laelaps chini from the endemic Hengduan Mountain rat species (Eothenomys miletus) and obtained the first complete mitochondrial genome of L. chini by next-generation sequencing (NGS). The L. chini mitogenome is 16,507 bp in size and contains 37 genes and a control region of 2380 bp in length. The L. chini mitogenome has a high AT content and a compact arrangement with four overlapping regions ranging from 1 to 2 bp and 16 spacer regions ranging from 1 to 48 bp. We analyzed 13 protein-coding genes of L. chini mitogenome and found that protein-coding genes in the L. chini mitogenome preferred codons ending in A/U and codon usage pattern was mainly influenced by natural selection. Cox1 has the slowest evolution rate and cox3 has the fastest evolution rate. We combined the mitochondrial genome of eight species of gamasid mites in the superfamily Dermanyssoidea from Genbank and the L. chini mitochondrial genome to analyze its rearrangement patterns and breakpoint numbers. We found that the L. chini mitogenome showed a novel arrangement pattern and nine species of gamasid mites in the superfamily Dermanyssoidea, which have been sequenced complete mitochondrial genomes to date, all showed different degrees of rearrangement. Laelaps chini, Echinolaelaps echidninus and Echinolaelaps fukinenensis were closely related species based on genetic distance and phylogenetic analyses. Notably they are clustered with Varroa destructor of the family Varroidae, suggesting that the family Varroidae is more closely related to the family Laelapidae, but more data are needed to test whether Varroa can be classified under the family Laelapidae. The L. chini mitogenome is the first complete mitochondrial genome for the genus Laelaps, and contributes to further exploration of the mitochondrial gene rearrangements and phylogeny for the superfamily Dermanyssoidea.
我们从特有横断山鼠种（Eothenomys miletus）中收集了 56 份中华革螨（Laelaps chini）标本，并通过下一代测序（NGS）获得了中华革螨的第一个完整线粒体基因组。中华革螨的线粒体基因组大小为 16,507 bp，包含 37 个基因和一个 2380 bp 长的控制区。中华革螨的线粒体基因组具有较高的 AT 含量和紧凑的排列，有四个重叠区长度为 1-2 bp，16 个间隔区长度为 1-48 bp。我们分析了中华革螨线粒体基因组的 13 个蛋白质编码基因，发现蛋白质编码基因在中华革螨线粒体基因组中偏好以 A/U 结尾的密码子，密码子使用模式主要受自然选择影响。Cox1 进化最慢，cox3 进化最快。我们将来自 Genbank 的 8 种革螨超家族 Dermanyssoidea 的线粒体基因组与中华革螨线粒体基因组相结合，分析其重排模式和断点数量。我们发现，中华革螨线粒体基因组表现出一种新的排列模式，而到目前为止已测序完成线粒体基因组的 8 种革螨超家族的物种都表现出不同程度的重排。基于遗传距离和系统发育分析，中华革螨、棘刺革螨（Echinolaelaps echidninus）和斐济棘刺革螨（Echinolaelaps fukinenensis）是密切相关的物种。值得注意的是，它们与蜂螨科（Varroidae）的瓦螨（Varroa destructor）聚类在一起，表明蜂螨科与革螨科更为接近，但需要更多的数据来检验瓦螨是否可以归类于革螨科。中华革螨的线粒体基因组是革螨属的第一个完整线粒体基因组，有助于进一步探索革螨超家族的线粒体基因重排和系统发育。
Zotero 插件