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Bacteroides eggerthii 酶 KdgF 的 H、C、N 共振赋值。

H, C, N resonance assignment of the enzyme KdgF from Bacteroides eggerthii.

机构信息

Department of Chemistry, DTU Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.

NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway.

出版信息

Biomol NMR Assign. 2022 Oct;16(2):343-347. doi: 10.1007/s12104-022-10102-6. Epub 2022 Aug 30.

DOI:10.1007/s12104-022-10102-6
PMID:36042150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9510102/
Abstract

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the H, C, and N NMR chemical shift assignment for BeKdgF is reported.

摘要

为了充分利用海藻生物质中的碳水化合物，必须了解被称为褐藻胶的多糖家族的降解过程。褐藻胶降解的一个步骤是由酶 KdgF 催化将 4,5-不饱和单糖醛酸转化为 4-脱氧-L-赤-5-己酮糖。在这项研究中，来自人类肠道微生物群的拟杆菌属中的 BeKdgF 在大肠杆菌中进行了同位素标记。本文报道了 BeKdgF 的 H、C 和 N NMR 化学位移归属。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/9be0b557876b/12104_2022_10102_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/75a7a5c983b9/12104_2022_10102_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/00f5dd033568/12104_2022_10102_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/9be0b557876b/12104_2022_10102_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/75a7a5c983b9/12104_2022_10102_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/00f5dd033568/12104_2022_10102_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/198e/9510102/9be0b557876b/12104_2022_10102_Fig3_HTML.jpg

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Proc Natl Acad Sci U S A. 2016 May 31;113(22):6188-93. doi: 10.1073/pnas.1524214113. Epub 2016 May 16.
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