Guangdong Provincial Key Laboratory for Plant Epigenetics; Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science; Longhua Institute of Innovative Biotechnology; College of Life Sciences and Oceanography, Shenzhen University, 518060 Shenzhen, Guangdong, China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, 518060 Shenzhen, Guangdong, China.
Front Biosci (Landmark Ed). 2022 Aug 19;27(8):251. doi: 10.31083/j.fbl2708251.
The plant hormones auxin affects most aspects of plant growth and development. The auxin transport and signaling are regulated by different factors that modulate plant morphogenesis and respond to external environments. The modulation of gene expression by Auxin Response Factors (ARFs) and inhibiting Auxin/Indole-3-Acetic Acid (Aux/IAA) proteins are involved in auxin signaling pathways. These components are encoded by gene families with numerous members in most flowering plants.
However, there is no genome-wide analysis of the expression profile and the structural and functional properties of the and gene families in soybean. Using various online tools to acquire of genomic and expression data, and analyzing them to differentiate the selected gene family's expression, interaction, and responses in plant growth and development.
Here, we discovered 63 and 51 in a genome-wide search for soybean and analyzed the genomic, sequential and structural properties of and . All of the found have the signature B3 DNA-binding (B3) and ARF (Aux rep) domains, with only 23 possessing the C-terminal PB1 (Phox and Bem1) domain (Aux/IAA), according to domain analysis. The number of exons in and genes varies from two to sixteen, indicating that the gene structure of and is highly variable. Based on phylogenetic analysis, the 51 GmARFs and 63 GmIAAs were classified into I-V and I-VII groups. The expression pattern of and revealed that the expression is more specific to a particular part of the plant; for example, , , and are highly expressed in the root. In contrast, expression has occurred in various parts of the plants. The interaction of ARF with functional genes showed extensive interactions with genes involved in auxin transport which helps to control plant growth and development. Furthermore, we also elaborate on the DNA-protein interaction of ARFs by identifying the residues involved in DNA recognition.
This study will improve our understanding of the auxin signaling system and its regulatory role in plant growth and development.
植物激素生长素影响植物生长和发育的各个方面。生长素的运输和信号转导受到不同因素的调节，这些因素调节植物形态发生并对外界环境做出反应。生长素响应因子 (ARF) 对基因表达的调节和抑制生长素/吲哚-3-乙酸 (Aux/IAA) 蛋白参与生长素信号通路。这些成分由具有许多成员的基因家族编码，在大多数开花植物中。
然而，在大豆中，生长素信号系统及其在植物生长发育中的调节作用的全基因组分析尚未进行。使用各种在线工具获取基因组和表达数据，并对其进行分析，以区分所选基因家族在植物生长和发育中的表达、相互作用和反应。
在这里，我们在大豆的全基因组搜索中发现了 63 个 和 51 个 ，并分析了 和 的基因组、序列和结构特性。根据结构域分析，所有发现的 都具有 B3 DNA 结合 (B3) 和 ARF (Aux rep) 结构域的特征，只有 23 个具有 C 末端 PB1 (Phox 和 Bem1) 结构域 (Aux/IAA)。 和 基因的外显子数量从两个到十六个不等，表明 和 的基因结构高度可变。基于系统发育分析，51 个 GmARFs 和 63 个 GmIAAs 被分为 I-V 和 I-VII 组。 和 的表达模式表明， 表达更特定于植物的特定部分；例如， 、 、和 高度表达于根中。相比之下， 表达发生在植物的各个部位。ARF 与功能基因的相互作用显示出与生长素运输相关的基因的广泛相互作用，这有助于控制植物的生长和发育。此外，我们还通过鉴定参与 DNA 识别的残基来详细阐述 ARF 的 DNA-蛋白质相互作用。
本研究将提高我们对生长素信号系统及其在植物生长和发育中的调节作用的理解。
