Unraveling the Guardians of Growth: A Comprehensive Analysis of the / and Gene Families in .

The auxin/indole-3-acetic acid () and auxin response factor () genes are two crucial gene families in the plant auxin signaling pathway. Nonetheless, there is limited knowledge regarding the and gene families in . In this study, we first identified 33 putative and 35 in the genome. Analysis of chromosomal location showed that the and were distributed unevenly across 17 chromosomes, with the greatest abundance observed on chromosomes 2. Furthermore, based on the homology of and , two phylogenetic trees were constructed, classifying 33 and 35 into three subgroups each. Five pairs of genes were identified as the outcome of tandem duplication, but no tandem repeat gene pairs were found in the family. The expression profiling of and revealed that several genes exhibited upregulation in different tissues and under various stress conditions, indicating their potential key roles in plant development and stress responses. The variance in expression patterns of specific and was corroborated through RT-qPCR analysis. Most importantly, we instituted that the gene, functioning as a central hub, exhibits interactions with numerous Aux/IAA and ARF proteins. Furthermore, subcellular localization findings indicate that functions as a protein localized within the nucleus. To conclude, the in-depth analysis provided in this study will contribute significantly to advancing our knowledge of the roles played by and families in both the development of tissue and its responses to stress. The insights gained will serve as a valuable asset for further inquiries into the biological functions of these gene families.