Qu Zhao-Lei, Li Xiao-Li, Ge Yan, Palviainen Marjo, Zhou Xuan, Heinonsalo Jussi, Berninger Frank, Pumpanen Jukka, Köster Kajar, Sun Hui
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
Department of Forest Sciences, University of Helsinki, Latokartanonkaari 7, P.O. Box 27, 00014, Helsinki, Finland.
Environ Microbiome. 2022 Aug 30;17(1):45. doi: 10.1186/s40793-022-00439-9.
Biochar is considered to be a possible means of carbon sequestration to alleviate climate change. However, the dynamics of the microbial community during wood decomposition after biochar application remain poorly understood. In this study, the wood-inhabiting bacterial community composition and its potential functions during a two-year decomposition period after the addition of different amounts of biochar (0.5 kg m and 1.0 kg m), and at different biochar pyrolysis temperatures (500 °C and 650 °C), in a boreal Scots pine forest, were analyzed using Illumina NovaSeq sequencing combined with Functional Annotation of Prokaryotic Taxa (FAPROTAX). The results showed that the wood decomposition rates increased after biochar addition to the soil surface in the second year. Treatment with biochar produced at high temperatures increased the diversity of wood-inhabiting bacteria more than that produced at low temperatures (P < 0.05). The wood-inhabiting bacterial diversity and species richness decreased with decomposition time. The biochar treatments changed the wood-inhabiting bacterial community structure during the decomposition period. The pyrolysis temperature and the amount of applied biochar had no effect on the bacterial community structure but shifted the abundance of certain bacterial taxa. Similarly, biochar application shifted the wood-inhabiting bacterial community function in the first year, but not in the second year. The wood-inhabiting bacterial community and function were affected by soil pH, soil water content, and soil total nitrogen. The results provide useful information on biochar application for future forest management practices. Long-term monitoring is needed to better understand the effects of biochar application on nutrient cycling in boreal forests.
生物炭被认为是一种可能的碳固存手段，以缓解气候变化。然而，生物炭施用后木材分解过程中微生物群落的动态变化仍知之甚少。在本研究中，利用Illumina NovaSeq测序结合原核生物分类功能注释（FAPROTAX），分析了在北方苏格兰松林中添加不同量生物炭（0.5 kg/m和1.0 kg/m）以及不同生物炭热解温度（500℃和650℃）后，木材栖息细菌群落组成及其在两年分解期内的潜在功能。结果表明，第二年在土壤表面添加生物炭后木材分解速率增加。高温制备的生物炭处理比低温制备的生物炭更能增加木材栖息细菌的多样性（P < 0.05）。木材栖息细菌的多样性和物种丰富度随分解时间而降低。生物炭处理在分解期改变了木材栖息细菌群落结构。热解温度和生物炭施用量对细菌群落结构没有影响，但改变了某些细菌类群的丰度。同样，生物炭施用在第一年改变了木材栖息细菌群落功能，但在第二年没有改变。木材栖息细菌群落和功能受土壤pH值、土壤含水量和土壤全氮的影响。研究结果为未来森林管理实践中生物炭的应用提供了有用信息。需要进行长期监测，以更好地了解生物炭施用对北方森林养分循环的影响。
