Xia Shulin, Ding Jiachen, Zhang Zhenhua, Li Xu, Gan Jianhe, He Xiaomin
Department of Infectious Disease, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.
Department of Infectious Disease, Affiliated Taixing People's Hospital of Yangzhou University, Taixing, People's Republic of China.
Infect Drug Resist. 2022 Aug 24;15:4837-4843. doi: 10.2147/IDR.S368392. eCollection 2022.
Studies have shown that cluster of differentiation (CD) 24 gene polymorphism is associated with several diseases. Among these, chronic hepatitis B (CHB) infection has not been investigated. This study aimed to assess the function of CD24 in CHB.
The study included 478 cases of CHB and 318 cases without CHB from 230 families that underwent genotyping. Polymerase chain reaction-restriction fragment length polymorphism was performed to assess the single nucleotide polymorphism (SNP) P170 of the CD24 gene. The detected genotypes were TT, CT, and CC. Then, family based-association analysis was carried out to investigate the association between CD24 gene polymorphism and susceptibility to CHB.
In the 478 patients with CHB, the frequencies of CD24 P170 T and C alleles were 35.5% and 64.5%, respectively, and the frequencies of CD24 P170 CC, CT, and TT genotypes were 39.3%, 50.4% and 10.3%, respectively. In a CD24 single-locus analysis by a family-based association test of P170 polymorphisms, T and C were not significantly associated with CHB in the additive ( = 0.169, = 0.866; = -0.169, = 0.866, respectively), dominant ( = 0.522, = 0.602; = 0.428, = 0.669, respectively), or recessive ( = -0.428, = 0.669; = -0.522, = 0.602, respectively) models. Transmission-disequilibrium (TD) and sib-transmission disequilibrium (STD) tests revealed no excess of T or C alleles from heterozygous parents to their children with the disease or higher frequencies of these alleles in patients compared with their normal siblings ( = 0.06, = 0.897).
The study findings suggest that the SNP P170 of CD24 has no significant association with susceptibility to the HB virus and related phenotypes in Chinese patients.
研究表明，分化簇（CD）24基因多态性与多种疾病相关。其中，慢性乙型肝炎（CHB）感染尚未得到研究。本研究旨在评估CD24在CHB中的作用。
该研究纳入了来自230个家庭的478例CHB患者和318例非CHB患者进行基因分型。采用聚合酶链反应-限制性片段长度多态性方法评估CD24基因的单核苷酸多态性（SNP）P170。检测到的基因型为TT、CT和CC。然后，进行基于家系的关联分析，以研究CD24基因多态性与CHB易感性之间的关联。
在478例CHB患者中，CD24 P170 T和C等位基因的频率分别为35.5%和64.5%，CD24 P170 CC、CT和TT基因型的频率分别为39.3%、50.4%和10.3%。在基于家系的P170多态性关联测试的CD24单基因座分析中，在加性模型（分别为χ² = 0.169，P = 0.866；χ² = -0.169，P = 0.866）、显性模型（分别为χ² = 0.522，P = 0.602；χ² = 0.428，P = 0.669）或隐性模型（分别为χ² = -0.428，P = 0.669；χ² = -0.522，P = 0.602）中，T和C与CHB均无显著关联。传递不平衡（TD）和同胞传递不平衡（STD）测试显示，杂合子父母向患病子女传递的T或C等位基因没有过量，且患者中这些等位基因的频率与正常同胞相比没有更高（χ² = 0.06，P = 0.897）。
研究结果表明，CD24的SNP P170与中国患者对HB病毒的易感性及相关表型无显著关联。
