Department of Plant Science, Faculty of Science, Shahrekord University, Shahrekord, Iran.
Department of Plant Science, Faculty of Science, Shahrekord University, Shahrekord, Iran; and Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
Funct Plant Biol. 2022 Nov;49(12):1055-1069. doi: 10.1071/FP21364.
In the present study, we used different LEDs to evaluate their effect on metabolic and transcriptional reprogramming of two genotypes (Ilam and Isfahan) of lemon balm grown under narrow-band LED lighting. Lemon balm plants were grown in four incubators equipped with artificial lighting and subjected to four LED lamps [White, Blue, Red, and mixed RB (Red+Blue) (70%:30%)] and in greenhouse conditions for 7weeks. The results showed significant increases in leaf number, pigment and soluble sugar contents, secondary metabolites, and calcium, magnesium, potassium and amino acid contents achieved in growth under mixed RB LEDs. As observed for the content of total phenolics, rosmarinic acid, and amino acids, the expression of genes involved in their production, including TAT , RAS , and DAHPS were also enhanced due to the mixed RB LED lighting. The best condition for both the plant growth and expression of genes was under the mixture of Red+Blue LED lamps. These observations indicate that the increase in secondary metabolites under mixed Red+Blue lights may be due to the increase in primary metabolites synthesis and the increased expression of genes that play an essential role in the production of secondary metabolites.
在本研究中，我们使用不同的 LED 来评估它们对窄带 LED 照明下生长的两种柠檬香脂基因型（Ilam 和 Isfahan）的代谢和转录重编程的影响。柠檬香脂植物在四个配备人工照明的培养箱中生长，并接受四种 LED 灯[白色、蓝色、红色和混合 RB（红+蓝）（70%：30%）]和温室条件下生长 7 周。结果表明，在混合 RB LED 下生长可显著增加叶片数、色素和可溶性糖含量、次生代谢物以及钙、镁、钾和氨基酸含量。如总酚、迷迭香酸和氨基酸含量所示，由于混合 RB LED 照明，参与其生产的基因的表达，包括 TAT、RAS 和 DAHPS，也得到了增强。对于植物生长和基因表达来说，红蓝混合 LED 灯是最佳条件。这些观察结果表明，混合红+蓝光照下次生代谢物的增加可能是由于初级代谢物合成的增加以及在次生代谢物生产中起重要作用的基因表达增加所致。
