Mansoury Fereshteh, Babaei Nahid, Abdi Soheila, Entezari Maliheh, Doosti Abbas
Department of Cell Biology and Genetics, Bushehr Branch, Islamic Azad University, Bushehr, Iran.
Department of Physics, Safadasht Branch, Islamic Azad University, Tehran, Iran. Email:
Cell J. 2022 Jul 27;24(7):364-369. doi: 10.22074/cellj.2022.7922.
Extremely low-frequency magnetic field (ELF-MF) exposure, as a targeted tumor therapy, presents several potential advantages. In this research, we investigated effects of different ELF-MF intensities on cell viability and expression levels of the mammalian target of rapamycin (mTOR) and hsa_circ_100338 in the normal fibroblast (Hu02) and human gastric adenocarcinoma (AGS) cell lines.
In this experimental study, cell lines of AGS and Hu02, were cultured under the exposure of ELFMF with magnetic flux densities (MFDs) of 0.25, 0.5, 1 and 2 millitesla (mT) for 18 hours. The 3-(4, 5-dimethylthiazoyl-2- yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cell viability. Relative expression of mTOR and hsa_circ_100338 RNAs was estimated by quantitative real-time polymerase chain reaction (qRT-PCR) technique.
Viability of the normal cells was significantly increased at MFDs of 0.5, 1 and 2 mT, while viability of the tumor cells was significantly decreased at MFD of 0.25 and increased at MFD of 2 mT. Expression level of mTOR was significantly increased at the all applied MFDs in the normal cells, while it was significantly decreased at MFDs of 0.25 and 0.5mT in the tumor cells. MFDs of 1 and 2 mT in tumor cells inversely led to the increase in mTOR expression. hsa_circ_100338 was downregulated in MFD of 0.25 mT and then it was increased parallel to the increase of MFD in the normal and tumor cells.
Results of the present study indicated that ELF-MF at MFDs of 0.25 and 0.5 mT can lead to decrease in the both mTOR and hsa_circ_100338 expression levels. Given the role of mTOR in cell growth, proliferation and differentiation, in addition to the potential role of hsa_circ_100338 in metastasis, expression inhibition of these two genes could be a therapeutic target in cancer treatment.
极低频磁场（ELF-MF）暴露作为一种靶向肿瘤治疗方法，具有若干潜在优势。在本研究中，我们调查了不同强度的ELF-MF对正常成纤维细胞（Hu02）和人胃腺癌细胞（AGS）系中细胞活力以及雷帕霉素哺乳动物靶点（mTOR）和hsa_circ_100338表达水平的影响。
在本实验研究中，AGS和Hu02细胞系在磁通密度（MFD）分别为0.25、0.5、1和2毫特斯拉（mT）的ELF-MF暴露下培养18小时。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估细胞活力。通过定量实时聚合酶链反应（qRT-PCR）技术估计mTOR和hsa_circ_100338 RNA的相对表达。
在MFD为0.5、1和2 mT时，正常细胞的活力显著增加，而在MFD为0.25 mT时肿瘤细胞的活力显著降低，在MFD为2 mT时肿瘤细胞活力增加。在所有施加的MFD下，正常细胞中mTOR的表达水平显著增加，而在肿瘤细胞中，MFD为0.25和0.5 mT时mTOR表达显著降低。肿瘤细胞中MFD为1和2 mT时，mTOR表达反而增加。在MFD为0.25 mT时hsa_circ_100338表达下调，然后在正常细胞和肿瘤细胞中随着MFD的增加而增加。
本研究结果表明，MFD为0.25和0.5 mT的ELF-MF可导致mTOR和hsa_circ_100338表达水平降低。鉴于mTOR在细胞生长、增殖和分化中的作用，以及hsa_circ_100338在转移中的潜在作用，抑制这两个基因的表达可能是癌症治疗的一个靶点。
