Rivera González Marco-Xavier, López de Mingo Isabel, Amuneke Ramírez Alexandra, Maestú Unturbe Ceferino
Laboratorio de Bioelectromagnetismo, Centro de Tecnología Biomédica (CTB), Universidad Politécnica de Madrid, Madrid, Spain.
Escuela Técnica Superior de Ingenieros Informáticos (ETSIINF), Universidad Politécnica de Madrid, Madrid, Spain.
Front Bioeng Biotechnol. 2024 Mar 8;12:1337899. doi: 10.3389/fbioe.2024.1337899. eCollection 2024.
In vitro studies requiring controlled exposure to low-frequency electromagnetic fields employ exposure systems with different geometries and configurations, the Helmholtz configuration being one of the most widely used. This configuration has limitations in the homogeneity of the spatial distribution of the magnetic field intensity values. We present the design, manufacturing, and characterisation of a new coil system, called RILZ configuration, which improves the distribution of magnetic field intensity values in the three dimensions of space for three different heights in comparison with the traditional circular coils in Helmholtz configuration. In addition, a comparative study of the cellular response in CT2A cultures exposed to a magnetic field of 50 Hz and 100 µT for 48 hrs is performed with both exposure systems. The results of the study show reduced values of deviation from the central value of magnetic field intensity using the RILZ coil system. These differences are statistically significant compared to the Helmholtz configuration for the three Cartesian directions: x ( < 0.01), y ( < 0.01), z ( < 0.01). In addition, the intensity values for three different heights are statistically significantly correlated using the RILZ coil system ( < 0.01). The differences in cell behaviour are also statistically significant between the two systems ( < 0.01) and may be directly related to the differences found in the distribution of intensity values between the two systems. This study highlights the importance of the homogeneity of the magnetic field intensity generated by the exposure systems used and offers an effective solution to control the magnetic field exposure parameters assays.
需要受控暴露于低频电磁场的体外研究采用具有不同几何形状和配置的暴露系统，亥姆霍兹配置是使用最广泛的配置之一。这种配置在磁场强度值的空间分布均匀性方面存在局限性。我们展示了一种名为RILZ配置的新型线圈系统的设计、制造和特性，与亥姆霍兹配置中的传统圆形线圈相比，该系统在三个不同高度的空间三维中改善了磁场强度值的分布。此外，使用这两种暴露系统对CT2A培养物暴露于50 Hz和100 µT磁场48小时后的细胞反应进行了比较研究。研究结果表明，使用RILZ线圈系统时，磁场强度中心值的偏差值降低。与亥姆霍兹配置相比，在笛卡尔坐标系的三个方向上：x（<0.01）、y（<0.01）、z（<0.01），这些差异具有统计学意义。此外，使用RILZ线圈系统时，三个不同高度的强度值具有统计学显著相关性（<0.01）。两种系统之间细胞行为的差异也具有统计学意义（<0.01），并且可能与两种系统之间强度值分布的差异直接相关。本研究强调了所用暴露系统产生的磁场强度均匀性的重要性，并为控制磁场暴露参数检测提供了有效解决方案。
