School of Dentistry, UniDomBosco University Center, Curitiba, Paraná, Brazil.
School of Dentistry, University of Tuiuti of Paraná, Curitiba, Paraná, Brazil.
Braz Dent J. 2022 Jul-Aug;33(4):12-20. doi: 10.1590/0103-6440202204963.
The study aimed to explore the influence of genetic polymorphisms in ANKK1 and DRD2 on the signs and symptoms of temporomandibular disorder (TMD) in construction workers. This cross-sectional study included only male subjects. All construction workers were healthy and over 18 years age. Illiterate workers and functionally illiterate workers were excluded. The diagnosis of TMD was established according to the Research Diagnostic Criteria for TMD (RDC/TMD). Genomic DNA was used to evaluate the genetic polymorphisms ANKK1 (rs1800497) and DRD2 (rs6275; rs6276) using Real-Time PCR. Chi-square or Fisher exact tests were used to evaluate genotypes and allele distribution among the studied phenotypes. The established alpha of this study was 5%. The sample included a total of 115 patients. The age of the patients ranged from 19 to 70 years (mean age 38.2; standard deviation 11.7). Chronic pain (87.7%), disc displacement (38.2%), and joint inflammation (26.9%) were the most frequently observed signs and symptoms. The genetic polymorphism rs6276 in DRD2 was associated with chronic pain (p=0.033). In conclusion, our study suggests that genetic polymorphisms in DRD2 and ANKK1 may influence TMD signs and symptoms in a group of male construction workers.
本研究旨在探讨 ANKK1 和 DRD2 基因多态性对建筑工人颞下颌关节紊乱症（TMD）体征和症状的影响。这是一项横断面研究，仅纳入男性受试者。所有建筑工人均为健康人群且年龄大于 18 岁。排除文盲和功能性文盲工人。TMD 的诊断根据 TMD 的研究诊断标准（RDC/TMD）确定。使用实时 PCR 评估 ANKK1（rs1800497）和 DRD2（rs6275；rs6276）的基因多态性。使用卡方或 Fisher 确切检验评估研究表型中的基因型和等位基因分布。本研究的建立 alpha 值为 5%。该样本共包括 115 名患者。患者年龄为 19 岁至 70 岁（平均年龄 38.2；标准差 11.7）。慢性疼痛（87.7%）、盘移位（38.2%）和关节炎症（26.9%）是最常见的体征和症状。DRD2 中的基因多态性 rs6276 与慢性疼痛相关（p=0.033）。总之，我们的研究表明，DRD2 和 ANKK1 基因多态性可能影响一组男性建筑工人的 TMD 体征和症状。
