青少年颞下颌关节紊乱症中的多巴胺受体 D2 和包含一个锚蛋白重复域的蛋白。

Dopamine receptor D2 and ankyrin repeat domain containing one in temporomandibular disorder of adolescents.

机构信息

School of Health Sciences, Universidade Positivo, Curitiba, Brazil.

Department of Stomatology, Universidade Federal do Paraná, Curitiba, Brazil.

出版信息

Int J Paediatr Dent. 2019 Nov;29(6):748-755. doi: 10.1111/ipd.12544. Epub 2019 Jun 11.

DOI:10.1111/ipd.12544

PMID:31144779

Abstract

BACKGROUND

Temporomandibular disorder (TMD) is a multifactorial condition that combines environmental and genetic factors and its prevalence increases during adolescence.

AIM

To investigate the association between TMD and genetic polymorphisms in the DRD2 and ANKK1 in a population of Brazilian adolescents.

DESIGN

The TMD group included adolescents diagnosed with any of the following TMD subgroups according to the RDC/TMD criteria: myofascial pain, arthralgia and disc displacement and painful TMD. Genomic DNA for molecular analysis was extracted from buccal cells, and genetic polymorphism rs6275 in DRD2 and rs1800497 in ANKK1 were genotyped by real-time polymerase chain reactions using the TaqMan assay. Data were analysed using the Epi Info 3.5.7 and Stata software, with significance level of 0.05.

RESULTS

Two hundred fifty-one individuals were included in this study, 148 subjects presented TMD. For disc displacement, the genetic polymorphisms rs6275 was associated in a recessive model (P = 0.04), whereas the rs6276 and rs1800497 presented only a borderline association in a recessive and dominant models, respectively (P = 0.07 and P = 0.06).

CONCLUSION

The genetic polymorphism rs6275 in DRD2 was associated with disc displacement in Brazilian adolescents.

摘要

背景

颞下颌关节紊乱（TMD）是一种多因素疾病，涉及环境和遗传因素，其患病率在青少年时期增加。

目的

调查巴西青少年人群中 DRD2 和 ANKK1 基因多态性与 TMD 的相关性。

设计

TMD 组包括根据 RDC/TMD 标准诊断为以下任何 TMD 亚组的青少年：肌筋膜疼痛、关节炎和关节盘移位以及疼痛性 TMD。从颊细胞中提取用于分子分析的基因组 DNA，通过 TaqMan 分析使用实时聚合酶链反应对 DRD2 中的 rs6275 和 ANKK1 中的 rs1800497 基因多态性进行基因分型。使用 Epi Info 3.5.7 和 Stata 软件分析数据，显著性水平为 0.05。