King Abdulaziz University, Department of Biological Sciences, Jeddah, Saudi Arabia.
Federal University Lokoja, Faculty of Sciences, Department of Biology, Lokoja, Kogi State, Nigeria.
Braz J Biol. 2022 Aug 29;84:e257145. doi: 10.1590/1519-6984.257145. eCollection 2022.
We studied the complete chloroplast genome of Gomphocarpus siniacus and Duvalia velutina from Asclepiadoideae subfamily; due to their medicinal importance and distribution worldwide their interest became high. In this study we analyzed the complete chloroplast genomes of G. siniacus and D. velutina using Illumina sequencing technology. The sequences were compared with the other species from Apocynaceae family. The complete genome of G. siniacus is 162,570 bp while D. velutina has154, 478 bp in length. Both genomes consist of 119 genes; encode 31 tRNA genes, and eight rRNA genes. Comparative studies of the two genomes showed variations in SSR markers in which G. siniacus possesses 223 while D. velutina has 186. This could be used for barcoding in order to aid in easy identification of the species. Phylogenetic analysis on the other hand reaffirms the tribal position of G. siniacus in Asclepiadeae and D. velutina in Ceropegieae. These findings could be used in subsequent research studies of angiosperms identification, genetic engineering, herb genomics and phylogenomic studies of Apocynaceae family.
我们研究了夹竹桃科萝藦亚科的球兰和吊灯花的完整叶绿体基因组;由于它们具有重要的药用价值和在世界范围内的分布，它们的研究兴趣变得很高。在这项研究中，我们使用 Illumina 测序技术分析了球兰和吊灯花的完整叶绿体基因组。这些序列与其他夹竹桃科的物种进行了比较。球兰的完整基因组长 162570bp，吊灯花的基因组长 154478bp。这两个基因组都由 119 个基因组成;编码 31 个 tRNA 基因和 8 个 rRNA 基因。对这两个基因组的比较研究显示，在 SSR 标记中存在差异，其中球兰有 223 个，吊灯花有 186 个。这可用于条形码，以帮助容易识别的物种。另一方面，系统发育分析证实了球兰在夹竹桃科中的部落地位和吊灯花在吊灯花科中的地位。这些发现可用于后续的被子植物鉴定、基因工程、草药基因组学和夹竹桃科的系统发育研究。
