基于催化发夹组装级联无金属 SPR 效应的超高灵敏度 miRNA-21 检测新型 ECL 传感系统。
A Novel ECL Sensing System for Ultrahigh Sensitivity miRNA-21 Detection Based on Catalytic Hairpin Assembly Cascade Nonmetallic SPR Effect.
机构信息
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China.
The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
出版信息
Anal Chem. 2022 Sep 13;94(36):12280-12285. doi: 10.1021/acs.analchem.2c03238. Epub 2022 Aug 31.
A novel electrochemical luminescence (ECL) sensing system is designed based on the catalytic hairpin assembly (CHA) cascade nonmetallic surface plasmon resonance (SPR) effect for ultrasensitive and selective miRNA-21 detection. In the CHA cycles, much more target miRNA-21 is captured to amplify the detection signal and enhance sensitivity. Meanwhile, the specific recognition between streptavidin (SA) and biotin, together with the SPR effect of SnS nanoplates on perylene tetracarboxylic acid, further amplifies the ECL signal. Together, miRNA-21 can be quantified with a limit of detection of 0.6 aM in the novel ECL system with ultrahigh sensitivity and selectivity. The ECL biosensor exhibits excellent stability and reproducibility as well as good practicality for miRNA-21 detection in real human serum.
一种新型的电化学发光（ECL）传感系统是基于催化发夹组装（CHA）级联非金属表面等离子体共振（SPR）效应设计的，用于超灵敏和选择性的 miRNA-21 检测。在 CHA 循环中，更多的靶标 miRNA-21 被捕获，以放大检测信号并提高灵敏度。同时，链霉亲和素（SA）与生物素之间的特异性识别，以及 SnS 纳米片对苝四羧酸的 SPR 效应，进一步放大了 ECL 信号。总之，该新型 ECL 系统可以实现对 miRNA-21 的定量检测，其检测限为 0.6 aM，具有超高的灵敏度和选择性。该 ECL 生物传感器表现出优异的稳定性和重现性，以及用于实际人血清中 miRNA-21 检测的良好实用性。
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