基于超高效液相色谱-高分辨质谱联用结合化学计量学的非靶向脂质组学方法用于五种蟹类的鉴别。
Untargeted Lipidomics Method for the Discrimination of Five Crab Species by Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry Combined with Chemometrics.
机构信息
National Demonstration Center for Experimental Ethnopharmacology Education, School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China.
Equipe de Chimie Analytique des Molécules Bioactives et Pharmacognoise, Institut Pluridisciplinaire Hubert Curien (UMR 7178, CNRS/UDS), 74 Route du Rhin, 67400 Illkirch, France.
出版信息
Molecules. 2023 Apr 22;28(9):3653. doi: 10.3390/molecules28093653.
In this study, ultra-high-performance liquid chromatography high-resolution accurate mass-mass spectrometry (UHPLC-HRAM/MS) was applied to characterize the lipid profiles of five crab species. A total of 203 lipid molecular species in muscle tissue and 176 in edible viscera were quantified. The results indicate that contained high levels of lipids with a docosahexaenoic acid (DHA) and eicosapntemacnioc acid (EPA) structure in the muscle tissue and edible viscera. A partial least squares discriminant analysis (PLS-DA) showed that PE 16:0/22:6, PE P-18:0/20:5, PA 16:0/22:6 and PC 16:0/16:1 could be used as potential biomarkers to discriminate the five kinds of crabs. In addition, some lipids, such as PE 18:0/20:5, PC 16:0/16:1, PE P-18:0/22:6 and SM 12:1;2O/20:0, could be used as characteristic molecules to distinguish between and , which are similar in appearance. This study provides a new perspective on discriminating crab species from MS-based lipidomics.
本研究采用超高效液相色谱-高分辨精确质量质谱（UHPLC-HRAM/MS）技术对 5 种蟹类的脂质谱进行了表征。共定量了肌肉组织中的 203 种脂质分子物种和可食内脏中的 176 种脂质分子物种。结果表明，肌肉组织和可食内脏中含有高水平的具有二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）结构的脂质。偏最小二乘判别分析（PLS-DA）表明，PE 16:0/22:6、PE P-18:0/20:5、PA 16:0/22:6 和 PC 16:0/16:1 可作为潜在的生物标志物来区分这 5 种蟹。此外，一些脂质，如 PE 18:0/20:5、PC 16:0/16:1、PE P-18:0/22:6 和 SM 12:1;2O/20:0，可作为区分外观相似的 和 的特征分子。本研究从基于 MS 的脂质组学角度为蟹类的物种鉴别提供了新的视角。
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