Jain Aditi, Gupta Arvind Kumar
IEEE/ACM Trans Comput Biol Bioinform. 2023 Mar-Apr;20(2):1600-1605. doi: 10.1109/TCBB.2022.3203171. Epub 2023 Apr 3.
We derive a deterministic mathematical model for the flow of ribosomes along a mRNA called the ribosome flow model with extended objects and abortions (RFMEOA). This model incorporates important cellular features such as every ribosome covers several codons and they may detach from various regions along the track due to more realistic biological situations including phenomena of ribosome-ribosome collisions. We prove that the ribosome density profile along the mRNA in the RFMEOA and in particular, the protein production rate converge to a unique steady-state. Simulations of the RFMEOA demonstrate a surprising result that an increase in the initiation rate may sometimes lead to a decrease in the production rate. We believe that this model could be helpful to provide insight into the effects of premature termination on the protein expression and be useful for understanding and re-engineering the translation process.
我们推导了一个关于核糖体沿信使核糖核酸（mRNA）流动的确定性数学模型，称为带有扩展对象和流产的核糖体流动模型（RFMEOA）。该模型纳入了重要的细胞特征，比如每个核糖体覆盖几个密码子，并且由于包括核糖体 - 核糖体碰撞现象在内的更现实的生物学情况，它们可能会沿着轨道从各个区域脱离。我们证明，RFMEOA中沿mRNA的核糖体密度分布，特别是蛋白质生产率会收敛到一个唯一的稳态。RFMEOA的模拟显示了一个惊人的结果，即起始速率的增加有时可能导致生产率的下降。我们认为这个模型有助于深入了解过早终止对蛋白质表达的影响，并且对于理解和重新设计翻译过程是有用的。
