Int J Periodontics Restorative Dent. 2022 Sep-Oct;42(5):e153-e159. doi: 10.11607/prd.5978.
This study evaluated in vitro the biologic profile of manually polished surfaces of pressed lithium disilicate (LD) ceramic compared to zirconia (Zir) in human gingival fibroblasts. Samples with a 10-mm diameter and 3-mm thickness were used. After manual polishing, the average roughness (Ra) of the samples was measured. The cell proliferation and viability of gingival fibroblasts on the surfaces were assessed at 24, 48, and 96 hours. Additionally, the morphology, cell adhesion, and type III collagen (COLIII) and vimentin (VIM) expression by fibroblasts plated onto these surfaces was analyzed. Polystyrene (Pol) was used as control for all assays. The mean Ra was 0.261 ± 0.053 μm for Zir and 0.345 ± 0.130 μm for LD. Both surfaces presented similar cell proliferation and viability (P > .05). The cell morphology demonstrated that, for both surfaces, the cells were occasionally spindle-shaped, parallel to the direction of the grooves. Compared to Pol, an upregulation of COLIII and VIM gene expression was observed by fibroblasts cultured on Zir and LD at all time points (P < .05). The characteristics presented by LD and Zir surfaces after manual polishing protocol were similar and had biologically favorable performances, thus suggesting LD as a suitable alternative to Zir in the peri-implant region for esthetic purposes.
本研究评估了在体外经手动打磨后的硅酸锂（LD）陶瓷与氧化锆（Zir）的生物学特性，比较其在人牙龈成纤维细胞中的表现。使用直径为 10mm、厚度为 3mm 的样本。手动打磨后，测量样本的平均粗糙度（Ra）。分别在 24、48 和 96 小时，评估牙龈成纤维细胞在表面的增殖和活力。此外，还分析了在这些表面上培养的成纤维细胞的形态、细胞黏附以及 III 型胶原蛋白（COLIII）和波形蛋白（VIM）的表达。所有实验均以聚苯乙烯（Pol）作为对照。Zir 的 Ra 平均值为 0.261 ± 0.053μm，LD 的 Ra 平均值为 0.345 ± 0.130μm。两种表面的细胞增殖和活力相似（P >.05）。细胞形态表明，对于两种表面，细胞偶尔呈纺锤形，与凹槽方向平行。与 Pol 相比，在所有时间点，培养在 Zir 和 LD 上的成纤维细胞的 COLIII 和 VIM 基因表达均上调（P <.05）。手动打磨方案后，LD 和 Zir 表面呈现出相似的特性，并具有良好的生物学性能，因此表明 LD 可作为在种植体周围区域用于美观目的的 Zir 的替代材料。
