Haustein J, Cruz-Sanchez F, Cervós-Navarro J
Zentralbl Allg Pathol. 1987;133(2):163-74.
We report on the ultrastructure of 21 ependymomas and propose the following diagnostic criteria: The presence of glycogen is the most constant phenomenon, followed by desmosomes, cilia, basal bodies and microvilli. Fifteen tumors had 3 or more of these criteria. The allegedly typical nuclear pattern was found in only 6 out of 21 cases. Special ultrastructural features of basement membranes were seen in a mid-thoracic spinal ependymoma, and two supratentorial tumors showed neurosecretory granules. Eleven cases were further studied with the PAP-method for demonstration of GFAP. All these cases were positive, GFAP was shown to be accumulated perivascularly. Positive cells were also found between the vessels. This distribution is characteristic for ependymomas.
我们报告了21例室管膜瘤的超微结构，并提出以下诊断标准：糖原的存在是最常见的现象，其次是桥粒、纤毛、基体和微绒毛。15个肿瘤符合3项或更多这些标准。在21例病例中，仅6例发现了所谓的典型核型。在一例胸段脊髓室管膜瘤中观察到基底膜的特殊超微结构特征，两例幕上肿瘤显示有神经分泌颗粒。对11例病例进一步采用PAP法检测胶质纤维酸性蛋白（GFAP）。所有这些病例均呈阳性，GFAP显示沿血管周围积聚。在血管之间也发现了阳性细胞。这种分布是室管膜瘤的特征。
