Kawano N, Yada K, Yagishita S
Department of Neurosurgery, Kitasato University School of Medicine, Kanagawa, Japan.
Virchows Arch A Pathol Anat Histopathol. 1989;415(5):467-72. doi: 10.1007/BF00747748.
Four cystic brain tumours, one from the frontal lobe, one in the third ventricle and two in the cerebellum, were studied histologically including immunostaining and electron microscopy. Anatomically, all the tumours were located within or adjacent to the ventricular system. By light microscopy, the tumours had a largely honeycomb pattern and were made up of clear cells. Some of the clear cells showed positive for GFAP. Leu 7, Factor VIII and NSE were negative. Electron microscopy of the areas with clear cells revealed densely packed polyhedral cells with clear cytoplasm. They had well developed intercellular junctions, microvilli and some cilia which confirmed their ependymal derivation. Perivascular pseudorosettes or papillary features were only seen in limited areas, where GFAP was strongly positive in the tumour cell processes. As these tumours mimic oligodendroglioma and cerebellar haemangioblastoma, they are called as "clear cell ependymoma" to obviate such errors in diagnosis.
对四个脑囊性肿瘤进行了组织学研究，包括免疫染色和电子显微镜检查，其中一个来自额叶，一个位于第三脑室，两个位于小脑。从解剖学上看，所有肿瘤均位于脑室系统内或其附近。光镜下，肿瘤主要呈蜂窝状，由透明细胞组成。部分透明细胞GFAP呈阳性。Leu 7、因子VIII和NSE均为阴性。对有透明细胞区域的电子显微镜检查显示，多面体细胞紧密排列，细胞质透明。它们具有发育良好的细胞间连接、微绒毛和一些纤毛，证实其来源于室管膜。仅在有限区域可见血管周围假菊形团或乳头状特征，肿瘤细胞突起中GFAP呈强阳性。由于这些肿瘤类似少突胶质细胞瘤和小脑成血管细胞瘤，故称为“透明细胞室管膜瘤”，以避免诊断中的此类错误。
