Liberski P P
Department of Oncology, School of Medicine, Lódź.
Folia Neuropathol. 1996;34(4):212-20.
I report here the ultrastructure of 29 ependymal tumors. The ultrastructural pattern was florid and characteristic with a picture dominated by the presence of microlumina, cilia with basal bodies (blepharoplast), microvilli and long, interdigitating intercellular junctions of the zonulae adherentes (adhesive plaque junctions) type. Tumor cells themselves were not particularly peculiar but they formed typical patterns of rosettes (so called mini- or ultrastructural rosettes) cell gatherings around small, electron-lucent lumina which are filled with numerous microvilli. Empty microlumina were rare. The apical and lateral portions of the cells surrounding microlumina were sealed by intercellular junctions which are long, tortuous and clearly different from the zonulae occludentes (tight junctions) of epithelial tumors. Clusters of apparently "redundant" junctions were occasionally visible comprising segments of different lengths. Ependymoma cells contained myriads of 10 nm intermediate filaments (glial filaments), occasionally forming thick bundles, virtually identical to those encountered in astrocytic tumors and forming an ultrastructural correlate for the GFAP immunostaining. The glycogen granules were often remarkably numerous. Numerous cilia, with a typical 9+1 pattern or with a distorted pattern were frequently observed in longitudinal or cross-sections.
在此我报告29例室管膜瘤的超微结构。超微结构模式丰富且具有特征性，其图像以微管腔、带有基体（生毛体）的纤毛、微绒毛以及紧密连接（粘着斑连接）类型的长而相互交错的细胞间连接的存在为主导。肿瘤细胞本身并无特别之处，但它们围绕小的、电子密度低的、充满大量微绒毛的管腔形成典型的玫瑰花结样（所谓的微或超微结构玫瑰花结）细胞聚集。空的微管腔很少见。围绕微管腔的细胞的顶端和侧面部分由细胞间连接封闭，这些连接长而曲折，明显不同于上皮性肿瘤的紧密连接。偶尔可见明显“多余”的连接簇，由不同长度的节段组成。室管膜瘤细胞含有无数10纳米的中间丝（神经胶质丝），偶尔形成粗束，与星形细胞瘤中所见的几乎相同，为GFAP免疫染色形成超微结构对应物。糖原颗粒通常非常多。在纵切面或横切面中经常观察到许多具有典型9 + 1模式或扭曲模式的纤毛。
