Grymel Mirosława, Mazurkiewicz Roman, Bajkacz Sylwia, Bilik Janusz, Kowalczyk Sławomir
Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Silesian University of Technology, Gliwice, Poland.
Department of Chemical Organic Technology and Petrochemistry, Silesian University of Technology, Gliwice, Poland.
Planta Med. 2023 Apr;89(5):551-560. doi: 10.1055/a-1903-2226. Epub 2022 Aug 31.
is an ethnobotanically significant plant with a relatiwely high content of spilanthol. Due to its broad spectrum of activity, including anti-inflammatory, antioxidant, analgesic, antifungal, and bacteriostatic properties, it is considered a valuable bioactive natural product. In addition, spilanthol as its main bioactive component inhibits facial muscle contractions, making it an attractive ingredient in anti-wrinkle and anti-aging cosmetics. Due to its muscle paralyzing effects, it is called herbal botox. The commercial interest in spilanthol encourages the development of effective methods of isolating it from plant material. The methodology used in this paper allows for the obtaining of extracts from with a relatively high content of spilanthol. An effective method of spilanthol extraction from all aerial parts of as well as methods of enriching spilanthol concentration in extracts achieved by removing polar and acidic substances from crude extracts was developed. To quantify the concentration of spilanthol, a simple, fast and economically feasible quantification protocol that uses nuclear magnetic resonance (HNMR) was developed. In addition, it has been proven, that oxidation of spilanthol by air gives (2,7)-6,9-endoperoxy--(2-methylpropyl)-2,7-decadienamide. The studies on spilanthol solutions stability were carried out and the conditions for the long-time storage of spilanthol solutions have also been developed. Additionally, for confirmation of obtained results a sensitive (LOQ=1 ng/mL), precise (RSD lower than 7%) and accurate (RE lower than 7.5%), new HPLC-MS/MS method was applied.
是一种在民族植物学上具有重要意义的植物，含有相对较高含量的毛喉鞘蕊花醇。由于其具有广泛的活性，包括抗炎、抗氧化、止痛、抗真菌和抑菌特性，它被认为是一种有价值的生物活性天然产物。此外，毛喉鞘蕊花醇作为其主要生物活性成分可抑制面部肌肉收缩，使其成为抗皱和抗衰老化妆品中一种有吸引力的成分。由于其肌肉麻痹作用，它被称为草本肉毒杆菌素。对毛喉鞘蕊花醇的商业兴趣促使人们开发从植物材料中分离它的有效方法。本文所采用的方法能够从[植物名称未明确]中获得含有相对较高含量毛喉鞘蕊花醇的提取物。开发了一种从[植物名称未明确]的所有地上部分提取毛喉鞘蕊花醇的有效方法，以及通过从粗提物中去除极性和酸性物质来提高提取物中毛喉鞘蕊花醇浓度的方法。为了定量毛喉鞘蕊花醇的浓度，开发了一种使用核磁共振（HNMR）的简单、快速且经济可行的定量方案。此外，已经证明，毛喉鞘蕊花醇在空气中氧化会生成(2,7)-6,9-内过氧基-(2-甲基丙基)-2,7-癸二烯酰胺。对毛喉鞘蕊花醇溶液的稳定性进行了研究，并制定了毛喉鞘蕊花醇溶液长期储存的条件。此外，为了确认所得结果，应用了一种灵敏（检测限=1 ng/mL）、精确（相对标准偏差低于7%）且准确（相对误差低于7.5%）的新型高效液相色谱-串联质谱法。
