Fabricius E M, Schmidt W, Schneeweiss U
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Mar;263(4):552-60. doi: 10.1016/s0176-6724(87)80199-2.
The microbiological tumour test is based on the detection of humoral antibodies to clostridial rods following intravenous injection of apathogenic clostridial spores. As serological methods we used the complement fixation test with a simultaneous short-time warm and long-time cold incubation as well as the passive haemagglutination test after absorption of clostridial group-specific antibodies. The evaluation of titre dynamics was rendered difficult by normal clostridial antibodies, by serological cross-reactions and, above all, by border-line reactions. The serological test results from 189 dogs investigated were evaluated in a multivariate analytical procedure. We determined the adequate combination of methods and antigens, thus improving the reliability of the test results and objectifying the predictive value of low sero-positive titres. On the basis of the computer-calculated individual weighting factors and the corresponding reference value it has become possible to evaluate and classify actual serological data emerging from the previous overall investigation.
微生物肿瘤检测基于静脉注射无致病性梭状芽孢杆菌孢子后检测针对梭状杆菌的体液抗体。作为血清学方法，我们使用了同时进行短时间温育和长时间冷育的补体结合试验，以及吸附梭状杆菌属特异性抗体后的被动血凝试验。正常的梭状杆菌抗体、血清学交叉反应，尤其是临界反应，使得对滴度动态的评估变得困难。我们采用多变量分析程序对189只受试犬的血清学检测结果进行了评估。我们确定了方法和抗原的适当组合，从而提高了检测结果的可靠性，并使低血清阳性滴度的预测价值更加客观。基于计算机计算的个体加权因子和相应的参考值，现在有可能对先前全面调查中产生的实际血清学数据进行评估和分类。
