Fabricius E M, Schneeweiss U, Schmidt W
Central Institute of Cancer Research, Academy of Sciences of the GDR, Berlin.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):99-112. doi: 10.1016/s0176-6724(87)80156-6.
The paper describes the development of appropriate antigen and method combinations for the microbiological cancer test using the non-oncolysing strain Clostridium butyricum CNRZ 528 in dogs with spontaneous tumours. The diagnostic rod antibodies could be determined quantitatively by the complement fixation test if a short-time warm fixation and a long-time cold fixation procedure were combined in separate runs and if two different antigens, a rod corpuscular antigen and a rod surface antigen, were used. Since complement-fixing antibodies were not always detected in cases of malignant tumours, we additionally used the passive haemagglutination method after pre-absorbing the sera with cross-reacting clostridial antigens. The efficiency of the microbiological cancer test could not be substantially increased, however, by the method combination the reliability of the evaluation of low seropositive titres was improved.
本文描述了使用非溶瘤性丁酸梭菌CNRZ 528对患有自发性肿瘤的犬进行微生物学癌症检测时，合适的抗原和方法组合的开发情况。如果在单独的实验中结合短时热固定和长时冷固定程序，并且使用两种不同的抗原，即杆状小体抗原和杆状表面抗原，那么可以通过补体结合试验定量测定诊断性杆抗体。由于在恶性肿瘤病例中并非总能检测到补体结合抗体，因此我们在用交叉反应性梭菌抗原预先吸收血清后，还使用了被动血凝法。然而，通过方法组合，微生物学癌症检测的效率并没有显著提高，但低血清阳性滴度评估的可靠性得到了改善。
