Zhang Huiyun, Hua Wenya, Lin Siran, Zhang Yu, Chen Xinchang, Wang Shiyong, Chen Jiazhen, Zhang Wenhong
Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
Infect Drug Resist. 2022 Aug 25;15:4845-4852. doi: 10.2147/IDR.S362583. eCollection 2022.
Nontuberculous mycobacteria (NTM) can cause pulmonary and extrapulmonary diseases. Tedizolid (TZD) is a new oxazolidinone with in vitro activity against NTM such as complex (MAC), , and complex. The aim of this study was to evaluate the TZD susceptibility profiles of clinical isolates of NTM.
The microdilution method was used to identify the minimum inhibitory concentration (MIC) of TZD and linezolid (LZD) for 133 clinical NTM isolates. Broth microdilution chequerboard assays were used to investigate the synergistic effects of TZD and three antibiotics on two reference isolates and eleven clinical isolates of NTM.
The TZD MIC and MIC for complex were 2 and 4 μg/mL, 16 and >32 μg/mL for MAC, respectively. TZD exhibited lower MICs than that of LZD for most NTM, which were positively correlated. Due to the high MIC values of TZD against MAC, it is necessary to conduct drug sensitivity tests before TZD administration. TZD-clarithromycin combination had synergistic response on complex in 3 of the 8 isolates, which lasted only 3-5 days. TZD-cefoxitin had synergistic effect against all five isolates.
Our study demonstrates that TZD had greater in vitro potency than LZD, and synergy studies suggested that TZD may be an important component of multi-drug treatment regimen.
非结核分枝杆菌（NTM）可引起肺部和肺外疾病。替地唑胺（TZD）是一种新型恶唑烷酮类药物，对鸟分枝杆菌复合群（MAC）等NTM具有体外活性。本研究旨在评估NTM临床分离株对TZD的药敏谱。
采用微量稀释法确定TZD和利奈唑胺（LZD）对133株NTM临床分离株的最低抑菌浓度（MIC）。采用肉汤微量稀释棋盘法研究TZD与三种抗生素对两株NTM参考菌株和11株临床分离株的协同作用。
TZD对鸟分枝杆菌复合群的MIC和MIC分别为2和4μg/mL，对MAC分别为16和>32μg/mL。对于大多数NTM，TZD的MIC低于LZD，且二者呈正相关。由于TZD对MAC的MIC值较高，在使用TZD前有必要进行药敏试验。TZD-克拉霉素联合用药对8株鸟分枝杆菌复合群菌株中的3株有协同反应，且仅持续3-5天。TZD-头孢西丁对所有5株脓肿分枝杆菌菌株均有协同作用。
我们的研究表明，TZD在体外的效力大于LZD，协同研究表明TZD可能是多药治疗方案的重要组成部分。
