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西汉墓葬出土陶俑表面真菌病害的分析与防治

Analysis and control of fungal deterioration on the surface of pottery figurines unearthed from the tombs of the Western Han Dynasty.

作者信息

Wang Yu, Wang Cen, Yang Xinyu, Ma Kaixuan, Guo Peifeng, Sun Qirui, Jia Shili, Pan Jiao

机构信息

Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.

Institute of Cultural Relics and Archaeology, Jinan, China.

出版信息

Front Microbiol. 2022 Aug 15;13:956774. doi: 10.3389/fmicb.2022.956774. eCollection 2022.

Abstract

In April 2020, 232 tombs of the Western Han Dynasty were found in Sundayuan, Heze City. In total, 141 pottery figurines of significant historical, cultural, and artistic value were unearthed from the tombs. Some of the figurines are currently being stored in warehouses, and the surface of some of the figurines show fungal deterioration. To thoroughly analyze the fungal deterioration on the surface of the pottery figurines and find appropriate control methods, we used high-through sequencing, scanning electron microscopy observation, pure cultures of culturable fungi, and optical microscopy observation and molecular identification of culturable fungi. We conducted fungistatic and simulation experiments in the laboratory to find appropriate control methods. We found that the fungi on the surface of the figurines were mainly of the phylum Ascomycota, and a few fungi were of the phyla Basidiomycota and Mucoromycota. We isolated seven culturable fungal strains and observed their colony morphology. The seven fungal strains were , , , sp., , , and . Through the fungistatic and simulation experiments conducted in the laboratory, we found that 50 mg/ml cinnamaldehyde and 0.5% K100 (2-methyl-4-isothiazolin-3-one) have a good fungistatic effect. They can not only inhibit the growth of fungi on medium, but also inhibit the growth of fungi on the surface of pottery figurines. This study has good reference significance for the analysis and control of fungal deterioration of unearthed pottery figurines.

摘要

2020年4月,菏泽市孙大园发现了232座西汉墓葬。这些墓葬共出土了141件具有重要历史、文化和艺术价值的陶俑。部分陶俑目前存放在仓库中,一些陶俑表面出现了真菌腐蚀现象。为深入分析陶俑表面的真菌腐蚀情况并找到合适的防治方法,我们采用了高通量测序、扫描电子显微镜观察、可培养真菌的纯培养以及可培养真菌的光学显微镜观察和分子鉴定。我们在实验室进行了抑菌和模拟实验以寻找合适的防治方法。我们发现陶俑表面的真菌主要属于子囊菌门,少数真菌属于担子菌门和毛霉门。我们分离出了7株可培养真菌菌株并观察了它们的菌落形态。这7株真菌菌株分别是 、 、 、 属、 、 、 。通过在实验室进行的抑菌和模拟实验,我们发现50毫克/毫升的肉桂醛和0.5%的K100(2-甲基-4-异噻唑啉-3-酮)具有良好的抑菌效果。它们不仅能抑制培养基上真菌的生长,还能抑制陶俑表面真菌的生长。本研究对出土陶俑真菌腐蚀的分析和防治具有良好的参考意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/218e/9421245/3ed6e553c110/fmicb-13-956774-g001.jpg

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