Wang Yu-Liang, Jian Xing, Wang Song
College of Life and Healthy Science, Anhui Science and Technology University, Bengbu, PR China.
College of Architecture, Anhui Science and Technology University, Bengbu, PR China.
Mitochondrial DNA B Resour. 2022 Aug 26;7(8):1550-1552. doi: 10.1080/23802359.2022.2111978. eCollection 2022.
Rupr. 1859 is a medicinal herb endemic to China and distributed throughout the country, particularly across the northern part of the mainland. However, the systematic classification of Euphorbiaceae remains controversial. Therefore, studying the chloroplast genome of is crucial for the resolution of this taxonomic dispute, clarification of the systematic status of , and establishment of an accurate classification system for Euphorbiaceae. In this study, we sequenced the complete chloroplast genome of using Illumina sequencing technology and annotated it using GeSeq. The complete chloroplast genome was 162,002-bp-long with a guanine-cytosine (GC) content of 35.7%. It included one large single-copy (LSC), one small single-copy (SSC), and two inverted repeat sequence regions (IRa and IRb), which were 90,225 bp, 18,067 bp, and 26,855 bp in length, respectively, and are indicative of a typical tetrad structure. The genome encoded 129 functional genes, comprising 85 protein-coding genes, 36 tRNA genes, and eight rRNA genes. According to the maximum-likelihood phylogenetic tree that was constructed using 16 complete chloroplast genomes, was found to be closely related to . Therefore, the complete chloroplast genome of provides a better understanding of genetics.
地锦草1859是中国特有的药用植物，分布于全国各地，尤其是中国大陆北部。然而，大戟科的系统分类仍存在争议。因此，研究地锦草的叶绿体基因组对于解决这一分类学争议、明确地锦草的系统地位以及建立准确的大戟科分类系统至关重要。在本研究中，我们使用Illumina测序技术对地锦草的完整叶绿体基因组进行了测序，并使用GeSeq进行注释。完整的叶绿体基因组长度为162,002 bp，鸟嘌呤-胞嘧啶（GC）含量为35.7%。它包括一个大单拷贝（LSC）、一个小单拷贝（SSC）和两个反向重复序列区域（IRa和IRb），长度分别为90,225 bp、18,067 bp和26,855 bp，呈现典型的四分体结构。该基因组编码129个功能基因，包括85个蛋白质编码基因、36个tRNA基因和8个rRNA基因。根据使用16个完整叶绿体基因组构建的最大似然系统发育树发现，地锦草与[具体物种]关系密切。因此，地锦草的完整叶绿体基因组有助于更好地了解地锦草的遗传学。
