Luo Xuan, Zhang Tongtong, Tang Hongliang, Liu Jiyang
Department of Chemistry, Key Laboratory of Surface and Interface Science of Polymer Materials of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, China.
Department of Hepatobiliary and Pancreatic Surgery, The Center for Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Front Nutr. 2022 Aug 15;9:962736. doi: 10.3389/fnut.2022.962736. eCollection 2022.
With the increasing application of antimicrobial peptides (AMPs) to replace antibiotics in medicine, food and agriculture, it is highly desired to develop a fast, reliable, and convenient strategy for sensitive detection of AMPs. Herein, a novel electrochemical (EC) and electrochemiluminescence (ECL) dual-modality sensing platform was developed based on probe encapsulated liposomes and nanochannel array modified electrodes, which enables sensitive determination of nisin in food samples. The bifunctional probe with both EC and ECL signals, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru(bpy) ), was chosen to be easily encapsulated in liposomes (Ru(bpy) @liposome). Based on the unique sterilization mechanism that AMPs can disrupt cell membranes, Ru(bpy) @liposome can be destroyed by nisin and release a large number of Ru(bpy) probes. Vertically-ordered mesoporous silica-nanochannel film (VMSF) modified ITO electrodes (VMSF/ITO) prepared by electrochemically assisted self-assembly (EASA) method were applied as the sensing electrode. Due to the efficient enrichment of Ru(bpy) by the negatively charged nanochannel arrays, VMSF/ITO enables detection of the EC/ECL signals of the released Ru(bpy) probes with ultrahigh sensitivity. In consequence, sensitive dual-modality detection of nisin was achieved by the combination of Ru(bpy) @liposome and VMSF/ITO. The developed sensing system can realize sensitive determination of nisin in ECL mode in the concentration range of 10 ng/ml to 50 μg/ml with a limit of detection (LOD) of 9.3 ng/ml, or in EC mode from 800 ng/ml to 100 μg/ml with a LOD of 70 ng/ml. Combined with the excellent anti-fouling and anti-interference performance of VMSF, rapid and sensitive detection of nisin in milk or egg white was also achieved by the sensor.
随着抗菌肽（AMPs）在医学、食品和农业领域中越来越多地用于替代抗生素，人们迫切需要开发一种快速、可靠且便捷的策略来灵敏检测AMPs。在此，基于探针包封脂质体和纳米通道阵列修饰电极开发了一种新型的电化学（EC）和电化学发光（ECL）双模态传感平台，可实现食品样品中乳链菌肽的灵敏测定。选择具有EC和ECL信号的双功能探针二氯三（2,2-联吡啶）钌（II）（Ru(bpy) ），以便于将其包封在脂质体中（Ru(bpy) @脂质体）。基于AMPs可破坏细胞膜的独特杀菌机制，Ru(bpy) @脂质体可被乳链菌肽破坏并释放大量Ru(bpy) 探针。通过电化学辅助自组装（EASA）方法制备的垂直有序介孔二氧化硅纳米通道膜（VMSF）修饰的ITO电极（VMSF/ITO）用作传感电极。由于带负电荷的纳米通道阵列对Ru(bpy) 的高效富集，VMSF/ITO能够以超高灵敏度检测释放的Ru(bpy) 探针的EC/ECL信号。因此，通过Ru(bpy) @脂质体和VMSF/ITO的组合实现了乳链菌肽的灵敏双模态检测。所开发的传感系统能够在ECL模式下实现对乳链菌肽的灵敏测定，其浓度范围为10 ng/ml至50 μg/ml，检测限（LOD）为9.3 ng/ml；或在EC模式下，浓度范围为800 ng/ml至100 μg/ml，LOD为70 ng/ml。结合VMSF优异的抗污染和抗干扰性能，该传感器还实现了牛奶或蛋清中乳链菌肽的快速灵敏检测。
