Chen Yuh-Ling, Huang Kuan-Chih, Wu Jer-Horng, Liu Tsunglin, Chen Jiung-Wen, Xie Jia-Yan, Chen Meng-Yen, Wu Li-Wha, Tung Chun-Liang
Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Department of Environmental Engineering, National Cheng Kung University, Tainan, Taiwan.
J Cancer. 2022 Aug 8;13(10):3051-3060. doi: 10.7150/jca.75947. eCollection 2022.
Oral cancer is one of the most common cancers worldwide and ranks fourth for the mortality rate of cancers in males in Taiwan. The oral microbiota is the microbial community in the oral cavity, which is essential for maintaining oral health, but the relationship between oral tumorigenesis and the oral microbiota remains to be clarified. This study evaluated the effect of microbiome dysbiosis on oral carcinogenesis in mice, and the impact of the microbiome and its metabolic pathways on regulating oral carcinogenesis. We found that antibiotics treatment decreases carcinogen-induced oral epithelial malignant transformation. Microbiome analysis based on 16S rRNA gene sequencing revealed that the species richness of fecal specimens was significantly reduced in antibiotic-treated mice, while that in the salivary specimens was not decreased accordingly. Differences in bacterial composition, including abundance, in the salivary samples of cancer-bearing mice was dramatically decreased. was the bacterial species that increased the most in the saliva of antibiotic-treated mice, suggesting that may be negatively associated with oral carcinogenesis. In functional analysis, the microbiome in the saliva of the tumor-bearing group showed greater potential for polyamine biosynthesis. Immunochemical staining proved that spermine oxidase, an effective polyamine oxidase, was upregulated in mouse oral cancer lesions. In conclusion, oral microbiome dysbiosis may alter polyamine metabolic pathways and reduce carcinogen-induced malignant transformation of the oral epithelium.
口腔癌是全球最常见的癌症之一，在台湾男性癌症死亡率中排名第四。口腔微生物群是口腔中的微生物群落，对维持口腔健康至关重要，但口腔肿瘤发生与口腔微生物群之间的关系仍有待阐明。本研究评估了微生物群失调对小鼠口腔致癌作用的影响，以及微生物群及其代谢途径对调节口腔致癌作用的影响。我们发现抗生素治疗可降低致癌物诱导的口腔上皮恶性转化。基于16S rRNA基因测序的微生物群分析显示，抗生素治疗的小鼠粪便标本的物种丰富度显著降低，而唾液标本中的物种丰富度并未相应降低。荷瘤小鼠唾液样本中细菌组成的差异，包括丰度，显著降低。 是抗生素治疗小鼠唾液中增加最多的细菌物种，表明 可能与口腔致癌作用呈负相关。在功能分析中，荷瘤组唾液中的微生物群显示出更大的多胺生物合成潜力。免疫化学染色证明，一种有效的多胺氧化酶精胺氧化酶在小鼠口腔癌病变中上调。总之，口腔微生物群失调可能会改变多胺代谢途径，降低致癌物诱导的口腔上皮恶性转化。
