• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

铁对顺铂耐药的上皮性卵巢癌细胞中钙相关基因表达水平的影响。

The effect of iron on the expression levels of calcium related gene in cisplatin resistant epithelial ovarian cancer cells.

作者信息

Kucukkaya Bahire, Erdag Demet, Akbas Fahri, Yalcintepe Leman

机构信息

Department of Biophysics, Faculty of Medicine, Istanbul Yeni Yuzyil University, 34010 Istanbul, Turkey.

Department of Computer programming, Vocational School, Biruni University, 34010 Istanbul, Turkey.

出版信息

Explor Target Antitumor Ther. 2021;2(4):309-322. doi: 10.37349/etat.2021.00048. Epub 2021 Aug 30.

DOI:10.37349/etat.2021.00048
PMID:36046755
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9400721/
Abstract

AIM

Anticancer drugs (chemotherapeutics) used in cancer treatment (chemotherapy) lead to drug resistance. This study was conducted to investigate the possible effect of iron on calcium homeostasis in epithelial ovarian cancer cells (MDAH-2774) and cisplatin-resistant cells of the same cell line (MDAH-2774/DDP).

METHODS

To develop MDAH-2774/DDP cells, MDAH-2774 (MDAH) cells were treated with cisplatin in dose increases of 5 μM between 0 μM and 70 μM. The effect of iron on the viability of MDAH and MDAH/DDP cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test at the end of 24 h incubation.

RESULTS

At increasing iron concentrations in MDAH and MDAH/DDP cells, the mRNA gene of fifteen genes [inositol 1,4,5-triphosphate receptor , ryanodine receptor , sarco/endoplasmic reticulum Ca ATPase , Na/Ca exchange , and plasma membrane Ca ATPase ] associated with Ca differences in expression were determined by quantitative reverse transcription-polymerase chain reaction. Changes in and gene expressions were observed in iron treatment of MDAH/DDP cells, while changes were detected in iron treatment of MDAH cells in and expressions.

CONCLUSIONS

This changes in the expression of calcium channels, pumps, and exchange proteins in the epithelial ovarian cancer cell line and in cisplatin-resistant epithelial ovarian cancer cells suggest that iron may have an important role in regulating calcium homeostasis. Due to differences in the expression of genes that play of an important role in the regulation of calcium homeostasis in the effect of iron, drug resistance can be prevented by introducing a new perspective on the use of inhibitors and activators of these genes and thus cytostatic treatment strategies.

摘要

目的

用于癌症治疗（化疗）的抗癌药物（化学治疗剂）会导致耐药性。本研究旨在探讨铁对上皮性卵巢癌细胞（MDAH - 2774）及同一细胞系的顺铂耐药细胞（MDAH - 2774/DDP）钙稳态的可能影响。

方法

为培养MDAH - 2774/DDP细胞，MDAH - 2774（MDAH）细胞用顺铂处理，顺铂剂量在0 μM至70 μM之间以5 μM的增幅递增。孵育24小时结束时，通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐试验测定铁对MDAH和MDAH/DDP细胞活力的影响。

结果

在MDAH和MDAH/DDP细胞中，随着铁浓度增加，通过定量逆转录 - 聚合酶链反应测定了与钙差异表达相关的15个基因[肌醇1,4,5 - 三磷酸受体、兰尼碱受体、肌浆/内质网钙ATP酶、钠/钙交换体和质膜钙ATP酶]的mRNA基因。在铁处理MDAH/DDP细胞时观察到[具体基因]和[具体基因]基因表达的变化，而在铁处理MDAH细胞时，在[具体基因]和[具体基因]表达中检测到变化。

结论

上皮性卵巢癌细胞系和顺铂耐药的上皮性卵巢癌细胞中钙通道、泵和交换蛋白表达的这种变化表明，铁可能在调节钙稳态中起重要作用。由于铁作用下在钙稳态调节中起重要作用的基因表达存在差异，通过引入关于这些基因抑制剂和激活剂使用的新视角，从而为细胞抑制治疗策略，可以预防耐药性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/b5a16aae7533/etat-02-100248-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/e15ad2e1b161/etat-02-100248-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/80fe01561172/etat-02-100248-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/5bbf9bbab52a/etat-02-100248-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/f4b35398e6e5/etat-02-100248-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/b5a16aae7533/etat-02-100248-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/e15ad2e1b161/etat-02-100248-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/80fe01561172/etat-02-100248-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/5bbf9bbab52a/etat-02-100248-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/f4b35398e6e5/etat-02-100248-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5546/9400721/b5a16aae7533/etat-02-100248-g005.jpg

相似文献

1
The effect of iron on the expression levels of calcium related gene in cisplatin resistant epithelial ovarian cancer cells.铁对顺铂耐药的上皮性卵巢癌细胞中钙相关基因表达水平的影响。
Explor Target Antitumor Ther. 2021;2(4):309-322. doi: 10.37349/etat.2021.00048. Epub 2021 Aug 30.
2
Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.顺铂耐药上皮性卵巢癌中的钙稳态
Gen Physiol Biophys. 2019 Jul;38(4):353-363. doi: 10.4149/gpb_2019013. Epub 2019 Jun 20.
3
Alteration of calcium homeostasis in primary preeclamptic syncytiotrophoblasts: effect on calcium exchange in placenta.原发性子痫前期合体滋养层细胞钙稳态的改变：对胎盘钙交换的影响。
J Cell Mol Med. 2011 Mar;15(3):654-67. doi: 10.1111/j.1582-4934.2010.01039.x.
4
[Effects of lentivirus-mediated heat shock protein 70 gene on endoplasmic reticulum calcium homeostasis and calcium channels of PC12 cells induced by ischemia and hypoxia].[慢病毒介导的热休克蛋白70基因对缺血缺氧诱导的PC12细胞内质网钙稳态及钙通道的影响]
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2016 Mar;28(3):205-10.
5
Curcumin induces apoptosis by inhibiting sarco/endoplasmic reticulum Ca2+ ATPase activity in ovarian cancer cells.姜黄素通过抑制卵巢癌细胞中的肌浆/内质网Ca2+ ATP酶活性诱导细胞凋亡。
Cancer Lett. 2016 Feb 1;371(1):30-7. doi: 10.1016/j.canlet.2015.11.021. Epub 2015 Nov 23.
6
Characterization of calcium signaling pathways in human preadipocytes.人类前脂肪细胞中钙信号通路的特征分析
J Cell Physiol. 2009 Sep;220(3):765-70. doi: 10.1002/jcp.21823.
7
Role of plasma membrane Ca2+-ATPase in contraction-relaxation processes of the bladder: evidence from PMCA gene-ablated mice.质膜Ca2+-ATP酶在膀胱收缩-舒张过程中的作用：来自质膜Ca2+-ATP酶基因敲除小鼠的证据。
Am J Physiol Cell Physiol. 2006 Apr;290(4):C1239-47. doi: 10.1152/ajpcell.00440.2005. Epub 2005 Nov 16.
8
Soluble CD40 ligands sensitize the epithelial ovarian cancer cells to cisplatin treatment.可溶性CD40配体使上皮性卵巢癌细胞对顺铂治疗敏感。
Biomed Pharmacother. 2016 Apr;79:166-75. doi: 10.1016/j.biopha.2016.01.006. Epub 2016 Feb 26.
9
Overexpression of P-glycoprotein in L1210/VCR cells is associated with changes in several endoplasmic reticulum proteins that may be partially responsible for the lack of thapsigargin sensitivity.P-糖蛋白在L1210/VCR细胞中的过表达与几种内质网蛋白的变化有关，这些变化可能部分导致了细胞对毒胡萝卜素缺乏敏感性。
Gen Physiol Biophys. 2008 Sep;27(3):211-21.
10
Regulation of sarco/endoplasmic and plasma membrane calcium ATPase gene expression by calcium in cultured human lens epithelial cells.培养的人晶状体上皮细胞中钙对肌浆网/内质网及质膜钙ATP酶基因表达的调控
Cell Calcium. 2007 Jan;41(1):87-95. doi: 10.1016/j.ceca.2006.05.003. Epub 2006 Jul 26.

引用本文的文献

1
Calcium signals and potential therapy targets in ovarian cancer (Review).钙信号与卵巢癌的潜在治疗靶点（综述）。
Int J Oncol. 2023 Nov;63(5). doi: 10.3892/ijo.2023.5573. Epub 2023 Sep 15.

本文引用的文献

1
Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.顺铂耐药上皮性卵巢癌中的钙稳态
Gen Physiol Biophys. 2019 Jul;38(4):353-363. doi: 10.4149/gpb_2019013. Epub 2019 Jun 20.
2
Lipid metabolism and Calcium signaling in epithelial ovarian cancer.上皮性卵巢癌中的脂代谢和钙信号转导。
Cell Calcium. 2019 Jul;81:38-50. doi: 10.1016/j.ceca.2019.06.002. Epub 2019 Jun 5.
3
Recent advances in understanding the ryanodine receptor calcium release channels and their role in calcium signalling.关于兰尼碱受体钙释放通道及其在钙信号传导中作用的最新研究进展。
F1000Res. 2018 Nov 27;7. doi: 10.12688/f1000research.16434.1. eCollection 2018.
4
Bcl-2 overexpression reduces cisplatin cytotoxicity by decreasing ER-mitochondrial Ca2+ signaling in SKOV3 cells.Bcl-2 过表达通过减少 SKOV3 细胞内质网-线粒体 Ca2+信号转导降低顺铂细胞毒性。
Oncol Rep. 2018 Mar;39(3):985-992. doi: 10.3892/or.2017.6164. Epub 2017 Dec 19.
5
Endoplasmic Reticulum-Mitochondria Calcium Communication and the Regulation of Mitochondrial Metabolism in Cancer: A Novel Potential Target.内质网-线粒体钙信号转导与癌症中线粒体代谢的调控：一个新的潜在靶点
Front Oncol. 2017 Sep 8;7:199. doi: 10.3389/fonc.2017.00199. eCollection 2017.
6
Calcium signaling and cell cycle: Progression or death.钙信号与细胞周期：前进或死亡。
Cell Calcium. 2018 Mar;70:3-15. doi: 10.1016/j.ceca.2017.07.006. Epub 2017 Jul 25.
7
Iron metabolism and drug resistance in cancer.癌症中的铁代谢与耐药性。
Biometals. 2017 Oct;30(5):629-641. doi: 10.1007/s10534-017-0037-7. Epub 2017 Aug 1.
8
Role of Na+/Ca2+ Exchangers in Therapy Resistance of Medulloblastoma Cells.钠/钙交换体在髓母细胞瘤细胞治疗耐药中的作用
Cell Physiol Biochem. 2017;42(3):1240-1251. doi: 10.1159/000478953. Epub 2017 Jul 3.
9
Alteration of sarcoplasmic reticulum Ca ATPase expression in lower limb ischemia caused by atherosclerosis obliterans.
Physiol Int. 2017 Jun 1;104(2):183-192. doi: 10.1556/2060.104.2017.2.1. Epub 2017 Jun 26.
10
Targeting Intracellular Calcium Signaling ([Ca]) to Overcome Acquired Multidrug Resistance of Cancer Cells: A Mini-Overview.靶向细胞内钙信号传导（[Ca]）以克服癌细胞获得性多药耐药性：简要概述
Cancers (Basel). 2017 May 9;9(5):48. doi: 10.3390/cancers9050048.