Graduate School of Human Development and Environment, Kobe University.
Department of Nutritional Science, Faculty of Nursing and Nutrition, University of Nagasaki.
J Nutr Sci Vitaminol (Tokyo). 2022;68(4):250-259. doi: 10.3177/jnsv.68.250.
This study investigated the effects of dietary high-dose biotin intake on fat oxidation in rats using respiratory gas analysis, and evaluated fatty-acid oxidation-related enzyme activities and gene expressions in the liver. Five-week-old male Sprague-Dawley rats were fed a control diet and three biotin-supplemented diets (additive biotin concentration: 0.05%, 0.10%, and 0.20% of diet) for 3 wk. In 2 wk, fat oxidation in the 0.20% biotin-supplemented diet group was higher than that in the 0.05% biotin-supplemented diet group; however, the energy expenditure and carbohydrate oxidation were unchanged between the dietary groups. At the end of 3 wk, body weight and epididymal white adipose tissue weight reduced in the 0.20% biotin diet group, and hepatic triglyceride levels tended to decrease. Additionally, increased plasma adiponectin concentration and hepatic mitochondrial carnitine palmitoyltransferase activity as well as decreased hepatic acetyl-CoA carboxylase 2 gene expression were observed in the 0.20% biotin-supplemented diet group compared with those in the control group. These results provide strong evidence that dietary high-dose biotin intake activated fat oxidation due to the increase in hepatic β-oxidation, which may contribute to the decrease in hepatic triglyceride concentration and white adipose tissue weight.
本研究通过呼吸气体分析，考察了高剂量生物素饮食对大鼠脂肪氧化的影响，并评估了肝脏中与脂肪酸氧化相关的酶活性和基因表达。将 5 周龄雄性 Sprague-Dawley 大鼠分别用对照饲料和 3 种添加生物素的饲料（生物素添加浓度：饲料的 0.05%、0.10%和 0.20%）喂养 3 周。在第 2 周，0.20%生物素添加饲料组的脂肪氧化高于 0.05%生物素添加饲料组；然而，各饲料组之间的能量消耗和碳水化合物氧化没有变化。在第 3 周结束时，0.20%生物素饮食组的体重和附睾白色脂肪组织重量降低，肝三酰甘油水平呈下降趋势。此外，与对照组相比，0.20%生物素添加饲料组的血浆脂联素浓度升高，肝线粒体肉碱棕榈酰转移酶活性升高，肝乙酰辅酶 A 羧化酶 2 基因表达降低。这些结果有力地证明，高剂量生物素饮食通过增加肝脏β氧化而激活脂肪氧化，这可能有助于降低肝三酰甘油浓度和白色脂肪组织重量。
