Department of Hepato-Biliary-Pancreatic and Transplant Surgery, Kyoto University.
Department of Gastroenterological and General Surgery, St Luke's International Hospital.
J Nutr Sci Vitaminol (Tokyo). 2022;68(4):276-283. doi: 10.3177/jnsv.68.276.
β-Hydroxy-β-methylbutyrate (HMB), a metabolite of leucine, is known to increase muscle mass and strength. However, the effect of perioperative HMB supplementation in liver surgery is unclear. Moreover, the impact of HMB on the skeletal muscle fiber type also remains unclear. We investigated the impact of HMB on the body composition and skeletal muscle fiber type in sarcopenic rats undergoing major hepatectomy. Nine-week-old male F344/NSlc rats were maintained in hindlimb suspension (HLS) and were forcedly supplemented with HMB calcium salt (HMB-Ca, 0.58 g/kg×2 times) or distilled water in addition to free feeding. After 2 wk of HLS, the rats underwent 70% hepatectomy and were sacrificed 3 d after surgery. Body composition factors and the proportion of slow-twitch fibers in hindlimb muscles were evaluated. HMB maintained the body composition and hindlimb force and acted against their deterioration in sarcopenic rats, exerting a particular effect on lean mass weight, which was significant. In the histological study, HMB significantly increased the proportion of slow-twitch fibers in the soleus (p=0.044) and plantaris (p=0.001) of sarcopenic rats. HMB ameliorated deterioration of the body composition and increased the proportion of slow-twitch fibers in sarcopenic rats undergoing major hepatectomy.
β-羟-β-甲基丁酸（HMB）是亮氨酸的代谢产物，已知可增加肌肉质量和力量。然而，围手术期 HMB 补充对肝手术的影响尚不清楚。此外，HMB 对骨骼肌纤维类型的影响也不清楚。我们研究了 HMB 对行大肝切除术的肌少症大鼠的身体成分和骨骼肌纤维类型的影响。9 周龄雄性 F344/NSlc 大鼠被维持在后肢悬吊（HLS）状态，并强制补充 HMB 钙盐（HMB-Ca，0.58 g/kg×2 次）或蒸馏水，同时自由进食。HLS 2 周后，大鼠接受 70%肝切除术，并在术后 3 天处死。评估身体成分因素和后肢肌肉中慢肌纤维的比例。HMB 维持了肌少症大鼠的身体成分和后肢力量，并防止其恶化，对瘦体重的影响尤为显著。在组织学研究中，HMB 显著增加了肌少症大鼠比目鱼肌（p=0.044）和跖肌（p=0.001）中慢肌纤维的比例。HMB 改善了肌少症大鼠大肝切除术后身体成分的恶化，并增加了慢肌纤维的比例。
