Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Jimo, QingDao City, Shandong Province 266237, China.
Department of Emergency, Qilu Hospital, Shandong University, Wen Hua Xi Lu, Jinan City, Shandong Province 250012, China.
Food Funct. 2022 Oct 3;13(19):9832-9846. doi: 10.1039/d2fo01715f.
Celery seeds are commonly used as condiments and in herbal teas with high medicinal value. In the present study, we investigated the contents of extracts derived under different extraction conditions and determined the optimal conditions for only extracting flavone glycosides from celery seeds. The compositional analysis identified three primary flavone glycosides in the ethanolic extract, and apiin, graveobioside A, and graveobioside B were isolated. Apigenin, luteolin, and chrsyeriol were obtained by the acid hydrolysis of flavone glycosides under high-temperature conditions. Here we investigated the inhibitory activity of apigenin and apiin on xanthine oxidase by reducing the rate of oxidative cytochrome C and found that both apigenin and apiin reduced cytochrome C production, except for low concentrations of apiin. analysis with hyperuricemia mice and rats showed that apiin had excellent uric acid-lowering effects and high dose-dependence, while apigenin was relatively slightly uric acid-lowering. In addition, the flavone glycoside extracts from celery seeds exhibited similar effects of reducing uric acid with apiin. Surprisingly, in hyperuricemia rats, the uric acid-lowering effects of high-dose apiin and flavone glycoside extracts were almost comparable to that of allopurinol. Besides, our experimental results showed that apigenin could improve uric acid clearance by increasing the glomerular filtration capacity, which was reflected in reducing the renal function parameters SUN and SCr; also, apiin showed better results. This study also showed that celery seeds have a unique medicinal value in treating hyperuricemia and that the flavone glycoside extracts from celery seeds can be developed as medicine for hyperuricemia.
芹菜籽通常用作调味品和草药茶，具有很高的药用价值。在本研究中，我们研究了不同提取条件下提取物的含量，并确定了仅从芹菜籽中提取黄酮糖苷的最佳条件。成分分析鉴定出乙醇提取物中有三种主要的黄酮糖苷，分离得到芹黄素、告依春苷 A 和告依春苷 B。通过高温条件下黄酮糖苷的酸水解得到芹菜素、木犀草素和 chrsyeriol。在这里，我们通过降低氧化细胞色素 C 的速率研究了芹黄素和芹黄素对黄嘌呤氧化酶的抑制活性，发现芹黄素和芹黄素都能降低细胞色素 C 的产生，除了低浓度的芹黄素。用高尿酸血症小鼠和大鼠进行分析表明，芹黄素具有极好的降尿酸作用和高剂量依赖性，而芹黄素的降尿酸作用相对较弱。此外，芹菜籽中的黄酮糖苷提取物具有与芹黄素相似的降低尿酸作用。令人惊讶的是，在高尿酸血症大鼠中，高剂量芹黄素和黄酮糖苷提取物的降尿酸作用几乎与别嘌醇相当。此外，我们的实验结果表明，芹黄素可以通过增加肾小球滤过率来提高尿酸清除率，这反映在降低肾功能参数 SUN 和 SCr 上；芹黄素也表现出更好的效果。本研究还表明，芹菜籽在治疗高尿酸血症方面具有独特的药用价值，芹菜籽中的黄酮糖苷提取物可开发为治疗高尿酸血症的药物。
