Hernández-Velázquez Ioreni Margarita, Zamora-Briseño Jesús Alejandro, Hernández-Bolio Gloria Ivonne, Hernández-Nuñez Emanuel, Lozano-Álvarez Enrique, Briones-Fourzán Patricia, Rodríguez-Canul Rossanna
Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Carr. Mérida-Progreso, CP 97310 Mérida, Yucatán, México.
Dis Aquat Organ. 2022 Sep 1;151:11-22. doi: 10.3354/dao03682.
Panulirus argus virus 1 (PaV1) (Family Mininucleoviridae) causes chronic and systemic infection in wild juvenile spiny lobsters Panulirus argus (Latreille, 1804), ending in death by starvation and metabolic wasting. In marine decapods, the antennal gland is involved in osmoregulation and excretion. In this compact organ, fluid is filtered from the hemolymph, and ions are reabsorbed to produce a hypotonic urine. Although PaV1 is released with the urine in infected individuals, little is known regarding the metabolic effect of PaV1 in the antennal gland. The objective of this study was to perform a comparative evaluation of the metabolic profile of the antennal gland of clinically PaV1-infected lobsters versus those with no clinical signs of infection, using proton nuclear magnetic resonance analysis. Overall, 48 compounds were identified, and the most represented metabolites were those involved in carbohydrate, amino acid, energy, and nucleotide metabolism. Most of the metabolites that were down-regulated in the infected group were essential and non-essential amino acids. Some metabolites involved in the urea cycle and carbohydrate metabolism were also altered. This study represents a first approach to the metabolic evaluation of the antennal gland. We broadly discuss alterations in the content of several proteinogenic and non-proteinogenic amino acids and other key metabolites involved in energetic and nucleotide metabolism.
锦绣龙虾病毒1（PaV1）（细小病毒科）可导致野生幼年锦绣龙虾（Panulirus argus，拉特雷尔，1804年）发生慢性全身性感染，最终因饥饿和代谢消耗而死亡。在海洋十足目动物中，触角腺参与渗透压调节和排泄。在这个紧凑的器官中，血液中的液体被过滤，离子被重新吸收以产生低渗尿液。虽然PaV1在受感染个体中随尿液排出，但关于PaV1在触角腺中的代谢作用知之甚少。本研究的目的是使用质子核磁共振分析，对临床感染PaV1的龙虾与无临床感染迹象的龙虾触角腺的代谢谱进行比较评估。总体而言，共鉴定出48种化合物，其中最具代表性的代谢物是那些参与碳水化合物、氨基酸、能量和核苷酸代谢的物质。感染组中下调的大多数代谢物是必需氨基酸和非必需氨基酸。一些参与尿素循环和碳水化合物代谢的代谢物也发生了改变。本研究是对触角腺代谢评估的首次尝试。我们广泛讨论了几种蛋白质氨基酸和非蛋白质氨基酸以及其他参与能量和核苷酸代谢的关键代谢物含量的变化。
