Grosman N, Hjort-Sørensen C, Jensen S M
Agents Actions. 1987 Apr;20(3-4):223-5. doi: 10.1007/BF02074675.
Human adenoids and tonsils were disintegrated mechanically and the cells dispersed by passage through a stainless-steel screen in EDTA-containing buffer. Collagenase digestion did not increase the yield of adenoidal cells. The mast cell content of the cell suspensions was in the range of 1-10 mast cells/10(4) cells with an estimated mean of 1-2 mast cells/10(4) cells, a value considerably below previous reports on adenoidal cell suspensions. The mast cell content was determined by staining with toluidine blue at low pH (to prevent interference by phagocytes). The mast cell count as assessed by alcian blue staining and by fluorescence microscopy after FITC-anti-human IgE binding was similar. Various attempts to enrich the cell suspension (i.e. by differential centrifugation, by gradient centrifugation on Ficoll or Ficoll-Hypaque and by velocity sedimentation at unit gravity) all gave negative results.
将人腺样体和扁桃体机械粉碎，细胞通过含乙二胺四乙酸（EDTA）缓冲液的不锈钢筛网分散。胶原酶消化并未提高腺样体细胞产量。细胞悬液中的肥大细胞含量为每10⁴个细胞含1 - 10个肥大细胞，估计平均值为每10⁴个细胞含1 - 2个肥大细胞，该值远低于先前关于腺样体细胞悬液的报道。通过在低pH值下用甲苯胺蓝染色（以防止吞噬细胞干扰）来测定肥大细胞含量。经阿尔辛蓝染色以及异硫氰酸荧光素（FITC）标记的抗人免疫球蛋白E（IgE）结合后用荧光显微镜评估的肥大细胞计数结果相似。为富集细胞悬液进行的各种尝试（即通过差速离心、在菲可（Ficoll）或菲可 - 泛影葡胺（Ficoll - Hypaque）上进行梯度离心以及在单位重力下进行速度沉降）均得到阴性结果。
