Halim H J, Fischer B, Schmutzler W
Agents Actions. 1982 Apr;12(1-2):189-91. doi: 10.1007/BF01965142.
Elutriation is a cell separation method based on countercurrent centrifugation. For mast cell purification it is superior to density gradient methods since no foreign protein or dense material has to be removed from the purified cells. The elutriation profiles of peritoneal rat mast cells differ considerably if the cells come from non-sensitized or actively sensitized donor animals and/or from different strains. This result is another indication of an altered morphological state of mast cells due to active immunization and is dependent on genetic factors. Cell aggregation proved to be the main obstacle to elutriation purification of guinea pig tissue mast cells. Human mast cells from adenoid tissues, however, could be brought to 70-90% purity, even if the elutriation profiles varied from donor to donor.
淘析是一种基于逆流离心的细胞分离方法。对于肥大细胞纯化而言，它优于密度梯度法，因为无需从纯化的细胞中去除外源蛋白质或致密物质。如果细胞来自未致敏或主动致敏的供体动物和/或不同品系，大鼠腹膜肥大细胞的淘析图谱会有很大差异。这一结果再次表明，主动免疫会使肥大细胞的形态状态发生改变，且这种改变依赖于遗传因素。细胞聚集被证明是豚鼠组织肥大细胞淘析纯化的主要障碍。然而，即使不同供体的淘析图谱有所不同，腺样组织中的人肥大细胞仍可达到70%-90%的纯度。
