Opt Lett. 2022 Sep 1;47(17):4552-4555. doi: 10.1364/OL.463087.
We present a unique super-resolution stimulated Raman scattering (SRS) microscopy technique based on phase-shifted spatial frequency modulation (PSFM) under wide-field illumination, permitting super-resolution chemical imaging with single-pixel detection. Through projecting a series of the pump and Stokes laser patterns with varying spatial frequencies onto the sample and combining with the proposed π-phase shift, the higher spatial information can be rapidly retrieved by implementing the fast inverse Fourier-transform on the spatial frequency-encoded SRS data. We have derived the theory of the PSFM-SRS technique for super-resolution imaging. Our further modeling results confirm that PSFM-SRS microscopy provides a ∼2.2-fold improvement in spatial resolution but with a much-reduced laser excitation power density required as compared with conventional point-scan SRS microscopy, suggesting its potential for label-free super-resolution chemical imaging in cells and tissue.
我们提出了一种独特的基于相移空间频率调制（PSFM）的宽场照明下的超分辨率受激拉曼散射（SRS）显微镜技术，可实现单像素检测的超分辨率化学成像。通过将一系列具有不同空间频率的泵浦和斯托克斯激光图案投影到样品上，并结合所提出的π相移，通过对空间频率编码的 SRS 数据进行快速逆傅里叶变换，可以快速提取更高的空间信息。我们已经推导出了用于超分辨率成像的 PSFM-SRS 技术的理论。我们进一步的建模结果证实，与传统的点扫描 SRS 显微镜相比，PSFM-SRS 显微镜在提供约 2.2 倍的空间分辨率提高的同时，所需的激光激发功率密度大大降低，这表明其在细胞和组织中的无标记超分辨率化学成像方面具有潜力。
