Tomaru T, Uchida Y, Masuo M, Kato A, Sugimoto T
Am Heart J. 1987 Jul;114(1 Pt 1):63-9. doi: 10.1016/0002-8703(87)90308-5.
We produced arterial thrombi that simulated the clinical condition in canine iliac arteries by endothelial denudation and partial occlusion. The processes of thrombus formation and thrombolysis were examined by vascular fiberscope. Laminar mural thrombi developed in all 21 preparations 10 minutes after blood reperfusion, with stenosis formation. Over 50% luminal obstruction with thrombi occurred in 10 preparations 30 minutes after inducing stenosis. Total occlusion with thrombi occurred in four preparation (31%) at 1 hour, and in all nine at 3 hours after inducing stenosis. Usually, red thrombus at the denudated region grew in size distal to the sited of stenosis. Histologically, thrombi of less than 1 hour duration consisted of a loose fibrin network and those of over 3 hours' duration consisted of a dense fibrin network. Infusion of 144,000 IU of urokinase (UK) reduced the size of thrombi less than 1 hour old during fiberoptic observation. However, UK infusion of similar dose failed to recanalize two of four preparations with 3-hour-old thrombi. In conclusion, arterial thrombi that closely simulate those observed clinically can be made by endothelial denudation and partial occlusion, and the vascular fiberscope provides a useful method for analysis of thrombosis and thrombolysis.
我们通过内皮剥脱和部分闭塞的方法，在犬髂动脉中制造出模拟临床状况的动脉血栓。采用血管纤维镜检查血栓形成和溶栓过程。在所有21个标本中，血液再灌注10分钟后形成了层状壁血栓，并伴有狭窄形成。在诱导狭窄30分钟后，10个标本中出现了血栓导致的管腔阻塞超过50%。诱导狭窄1小时后，4个标本（31%）出现血栓完全阻塞，3小时后所有9个标本均出现完全阻塞。通常，剥脱区域的红色血栓在狭窄部位远端增大。组织学上，持续时间小于1小时的血栓由疏松的纤维蛋白网络组成，持续时间超过3小时的血栓由致密的纤维蛋白网络组成。在纤维镜观察期间，输注144,000国际单位的尿激酶（UK）可减小持续时间小于1小时的血栓大小。然而，输注相同剂量的UK未能使4个有3小时血栓的标本中的2个再通。总之，通过内皮剥脱和部分闭塞可制造出与临床观察到的血栓非常相似的动脉血栓，血管纤维镜为分析血栓形成和溶栓提供了一种有用的方法。
