Uchida Y, Masuo M, Tomaru T, Kato A, Sugimoto T
Am Heart J. 1986 Oct;112(4):691-6. doi: 10.1016/0002-8703(86)90462-x.
Coronary arteries isolated from cadavers autopsied within 7 hours after death were perfused with canine arterial blood, and the processes of thrombus formation at the segments stenosed with atheroma and the thrombolytic effects of urokinase were investigated by angioscopy. Ten minutes of blood perfusion caused thin mural thrombi localized at the stenotic or nonstenotic segments. During 30 minutes of blood perfusion, the thin mural thrombi of the outlet or inlet of the segment grew into a doughnut-shaped thrombus. Also, the thin mural thrombi in the stenotic segment grew into a streamer-like thrombus and drifted downstream. These thrombi grew in size with increasing perfusion time and finally obstructed the stenotic segment. Globular thrombi close to the outlet also were formed in a few preparations. Unlike the thrombi at the stenotic segment, the mural thrombi in the nonstenotic segments did not grow into massive thrombi. The thrombi were reduced in size within 10 minutes of perfusion with 320 U/ml or more of urokinase. During thrombolysis, sandstorm-like dispersion of the blood cells occurred, small fragments detached from the mother thrombus and flew downstream, or the fibrin core of the thrombus was exposed. The results indicate the usefulness of angioscopy for the dynamic and serial investigation of thrombosis and thrombolysis.
对死后7小时内尸检的尸体分离出的冠状动脉用犬动脉血进行灌注，并通过血管镜检查研究动脉粥样硬化狭窄节段的血栓形成过程以及尿激酶的溶栓作用。血液灌注10分钟后，在狭窄或非狭窄节段出现薄的壁血栓。血液灌注30分钟时，节段出口或入口处的薄壁血栓长成环形血栓。此外，狭窄节段的薄壁血栓长成飘带状血栓并向下游漂移。这些血栓随着灌注时间的延长而增大，最终阻塞狭窄节段。在一些标本中还形成了靠近出口的球形血栓。与狭窄节段的血栓不同，非狭窄节段的壁血栓不会长成大块血栓。用320 U/ml或更高浓度的尿激酶灌注10分钟内血栓体积减小。溶栓过程中，血细胞出现沙尘暴样弥散，小碎片从母血栓上脱落并向下游飞散，或血栓的纤维蛋白核心暴露。结果表明血管镜检查对血栓形成和溶栓的动态及连续研究有用。
