丙烯腈的致突变性。

Mutagenicity of acrylonitrile.

作者信息

de Meester C, Poncelet F, Roberfroid M, Mercier M

出版信息

Toxicology. 1978 Sep;11(1):19-27. doi: 10.1016/s0300-483x(78)90239-1.

Abstract

Incubation of Salmonella typhimurium strains in an atmosphere of 0.2% gaseous acrylonitrile increased the numbers of his+ revertants/plate only in the presence of a fortified S9 liver fraction. The mutagenic effect was particularly pronounced with strains TA1530, TA1535 and TA1950 and much weaker with strains TA100, TA98 and TA1978. The results of bacterial fluctuation tests confirmed the necessity of the presence of S9 mix and showed the particular sensitivity of TA1530. The reversion rate varied with the S9 mix composition, the animal species utilized and the type of pretreatments applied to the animals. The mutagenicity of acrylonitrile in S. typhimurium is therefore microsome-mediated and is particularly discernable with strains sensitive to base-substitution mutagens.

摘要

将鼠伤寒沙门氏菌菌株置于含有0.2%气态丙烯腈的环境中培养，只有在添加了强化S9肝匀浆的情况下，每平皿中his+回复突变体的数量才会增加。这种诱变效应在TA1530、TA1535和TA1950菌株中尤为明显，而在TA100、TA98和TA1978菌株中则弱得多。细菌波动试验的结果证实了S9混合物存在的必要性，并显示出TA1530的特殊敏感性。回复突变率随S9混合物的组成、所用动物物种以及对动物进行的预处理类型而变化。因此，丙烯腈在鼠伤寒沙门氏菌中的诱变性是由微粒体介导的，并且在对碱基置换诱变剂敏感的菌株中尤为明显。