School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China.
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China.
Bioresour Technol. 2022 Oct;362:127874. doi: 10.1016/j.biortech.2022.127874. Epub 2022 Aug 30.
The sulfonamide antibiotic resistance genes (ARGs) especially sul1 was identified as the dominant in eutrophic water. The performance of Chlorella vulgaris-B. licheniformis consortium toward sul1 removal, total nitrogen (TN) removal, and the mechanism of sul1 removal was investigated. The removal efficiency of exogenous ARGs plasmids carrying sul1 reached (97.2 ± 2.3)%. The TN removal rate reached (98.5 ± 1.2)%. The enhancements of carbon metabolism, nitrogen metabolism, aminoacyl-tRNA biosynthesis, and glycoproteins had significant influences on sul1 and TN removals, under the premise of normal growth of algae and bacteria. The quantitative polymerase chain reaction (qPCR) results suggested that the absolute abundances of sul1 were low in algal-bacterial systems (0 gene copies/mL) compared with individual systems ((1 × 10 ± 15) gene copies/mL). The duplication of sul1 was inhibited in algal cells and bacterial cells. The algal-bacterial consortium seems to be a promising technology for wastewater treatment with a potential to overcome the eutrophication and ARGs challenges.
在富营养化水中，磺胺类抗生素抗性基因（ARGs），特别是 sul1，被确定为主要的。研究了小球藻-地衣芽孢杆菌联合体对 sul1 去除、总氮（TN）去除的性能及其去除机制。携带 sul1 的外源 ARGs 质粒的去除效率达到（97.2±2.3）%。TN 去除率达到（98.5±1.2）%。在藻类和细菌正常生长的前提下，碳代谢、氮代谢、氨酰-tRNA 生物合成和糖蛋白的增强对 sul1 和 TN 的去除有显著影响。定量聚合酶链反应（qPCR）结果表明，与单一系统相比，藻类-细菌系统中 sul1 的绝对丰度较低（0 个基因拷贝/mL）（（1×10±15）个基因拷贝/mL）。sul1 的复制在藻类细胞和细菌细胞中受到抑制。藻类-细菌联合体似乎是一种很有前途的废水处理技术，具有克服富营养化和 ARGs 挑战的潜力。
