Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore.
Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore.
Sci Rep. 2022 Sep 1;12(1):14895. doi: 10.1038/s41598-022-18309-2.
Transplacental docosahexaenoic-acid (DHA) supply for fetal development is regulated by placental DHA-lipid metabolism. Both maternal diabetes and obesity are linked to possible decreased fetal circulating DHA and increased placental DHA-lipids. Since myo-inositol is a promising intervention for gestational diabetes (GDM), we aimed to determine whether myo-inositol could rectify perturbations in placental DHA metabolism associated with maternal increasing glycemia and obesity and examine links with birthweight. Term placental villous explants from 17 women representing a range of BMIs and mid-gestational glycemia, were incubated with C-labeled-DHA for 48 h, in 0.3 µmol/L (control) or 60 µmol/L myo-inositol. Individual newly synthesized C-DHA-labeled lipid species were quantified by liquid-chromatography-mass-spectrometry. Compared with controls, incubation with myo-inositol decreased most C-DHA-lipids in placental explants from women with higher BMI or higher glycemia, but increased C-DHA-lipids with normal BMI or lower glycemia. Myo-inositol also increased C-DHA-labeled lipids in cases of lower birthweight centile, but induced decreases at higher centiles. Myo-inositol therefore lowered DHA-lipids in placenta with high basal placental DHA-lipid production (higher BMI and glycemia) but increased DHA-lipids where basal processing capacity is low. Myo-inositol thus moderates placental DHA metabolism towards a physiological mean which may in turn moderate birthweight.
胎盘源性二十二碳六烯酸（DHA）供应受胎盘 DHA 脂质代谢调控。母体糖尿病和肥胖均与胎儿循环 DHA 减少和胎盘 DHA 脂质增加相关。由于肌醇是治疗妊娠期糖尿病（GDM）的一种有前途的干预措施，我们旨在确定肌醇是否可以纠正与母体血糖升高和肥胖相关的胎盘 DHA 代谢紊乱，并研究其与出生体重的关联。将代表一系列 BMI 和妊娠中期血糖的 17 名女性的胎盘绒毛外植体与 C 标记的 DHA 在 0.3 µmol/L（对照）或 60 µmol/L 肌醇中孵育 48 小时。通过液相色谱-质谱法对新合成的 C-DHA 标记脂质种类进行定量。与对照组相比，在高 BMI 或高血糖的女性中，肌醇孵育降低了胎盘外植体中大多数 C-DHA 脂质，但增加了 BMI 正常或血糖较低的 C-DHA 脂质。肌醇还增加了出生体重百分位数较低的情况下的 C-DHA 标记脂质，但在较高百分位数时诱导了降低。因此，肌醇降低了基础胎盘 DHA 脂质产量较高（BMI 和血糖较高）的胎盘 DHA 脂质，但增加了基础处理能力较低的胎盘 DHA 脂质。因此，肌醇使胎盘 DHA 代谢向生理平均值调节，这反过来又可能调节出生体重。
