Cao Liang, Qin Bin, Gong Zhenping, Zhang Yuxian
College of Agriculture, Northeast Agricultural University, Harbin, 150006 Heilongjiang China.
Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing, China.
Physiol Mol Biol Plants. 2022 Jul;28(7):1477-1488. doi: 10.1007/s12298-022-01219-y. Epub 2022 Aug 19.
Drought affects the normal growth and development of soybeans. Melatonin reportedly alleviates drought stress-induced growth inhibition and plant injury, thus, its foliar application presumably has considerable potential in agriculture. However, few studies have investigated the mechanism responsible for its effects on soybean nitrogen metabolism. In this study, pot culture and plant physiological detection, qPCR, and other methods were used for analysis. The purpose of this study was to explore the effects of melatonin and melanin on glutathione metabolism. The results showed that drought stress led to an increase in soluble protein and proline content, concomitantly with a decrease in the activity of nitrogen metabolism-related key enzymes, an increase in inorganic nitrogen content, and a reduction in nitrogen accumulation and transport. Exogenous melatonin application under drought stress significantly increased the expression of key genes involved in nitrogen metabolism and the activity of key enzymes including, GOGAT, NR, Gs and GDH. Enhanced enzyme activity promotes the conversion of nitrate nitrogen in plants, increases proline, soluble protein, and ureide contents, and, consequently, nitrogen accumulation. Altogether, these changes were conducive to greater nitrogen assimilation and transport. Therefore, under drought stress, melatonin application upregulated key genes involved in nitrogen metabolism, thereby enhancing the activity of related enzymes and restoring growth, stable biomass production.
The online version contains supplementary material available at 10.1007/s12298-022-01219-y.
干旱影响大豆的正常生长发育。据报道，褪黑素可缓解干旱胁迫诱导的生长抑制和植物损伤，因此，其叶面喷施在农业上可能具有相当大的潜力。然而，很少有研究探讨其对大豆氮代谢影响的作用机制。在本研究中，采用盆栽培养、植物生理检测、qPCR等方法进行分析。本研究的目的是探讨褪黑素和黑色素对谷胱甘肽代谢的影响。结果表明，干旱胁迫导致可溶性蛋白和脯氨酸含量增加，同时氮代谢相关关键酶活性降低，无机氮含量增加，氮素积累和转运减少。干旱胁迫下外源喷施褪黑素显著增加了氮代谢相关关键基因的表达以及关键酶（包括谷氨酰胺合成酶、硝酸还原酶、谷氨酸合成酶和谷氨酸脱氢酶）的活性。酶活性增强促进了植物体内硝态氮的转化，增加了脯氨酸、可溶性蛋白和酰脲含量，从而促进了氮素积累。总之，这些变化有利于提高氮素同化和转运。因此，在干旱胁迫下，喷施褪黑素上调了氮代谢相关关键基因，从而增强了相关酶的活性，恢复了生长，稳定了生物量生产。
网络版包含可在10.1007/s12298-022-01219-y获取的补充材料。
