Faculty of Agriculture, Northeast Agricultural University, Xiangfang District, Harbin, 150030, China.
Agriculture and Food Science and Technology Branch, Heilongjiang Agricultural Engineering Vocational College, Nangang District, Harbin, 150025, China.
Sci Rep. 2023 Jul 5;13(1):10911. doi: 10.1038/s41598-023-38088-8.
As an important bioactive molecule, nitric oxide (NO) can effectively alleviate the effects of drought stress on crops. However, it is still unclear whether it can increase the stress resistance of soybean. Therefore, in this study, our objective was to explore the effect of exogenous NO application on the physiological characteristics of soybean seedlings under drought stress. As test material, two soybean varieties, HN65 and HN44, were used, while sodium nitroprusside (SNP) of 100 μmol L, 200 μmol L, 500 μmol L, 1000 μmol L served as an exogenous NO donor, and PEG-6000 as an osmotic regulator to simulate drought stress. The effects of irrigation with different SNP concentrations for different days on the physiological characteristics of the soybean seedlings under drought conditions were then investigated. The results obtained showed that the activities of antioxidant enzymes, osmotic regulator contents, as well as the abscisic acid and salicylic acid contents of the plant leaves increased with increasing SNP concentration and treatment time. However, we observed that excessively high SNP concentrations decreased the activities of key nitrogen metabolism enzymes significantly. This study provides a theoretical basis for determining a suitable exogenous NO concentration and application duration. It also highlights strategies for exploring the mechanism by which exogenous NO regulates crop drought resistance.
作为一种重要的生物活性分子，一氧化氮（NO）可以有效缓解干旱胁迫对作物的影响。然而，目前尚不清楚它是否能提高大豆的抗逆性。因此，在本研究中，我们的目的是探讨外源 NO 处理对干旱胁迫下大豆幼苗生理特性的影响。以 HN65 和 HN44 两个大豆品种为试验材料，以 100μmol·L-1、200μmol·L-1、500μmol·L-1、1000μmol·L-1 的硝普钠（SNP）为外源 NO 供体，以 PEG-6000 为渗透调节剂模拟干旱胁迫。然后研究了不同 SNP 浓度和处理时间对干旱条件下大豆幼苗生理特性的影响。结果表明，随着 SNP 浓度和处理时间的增加，抗氧化酶活性、渗透调节物质含量以及叶片中脱落酸和水杨酸含量增加，但是过高的 SNP 浓度会显著降低关键氮代谢酶的活性。本研究为确定适宜的外源 NO 浓度和施用时间提供了理论依据，也为探索外源 NO 调节作物抗旱性的机制提供了策略。
