Isolation and biosynthesis of daturamycins from sp. KIB-H1544.

Two novel diarylcyclopentenones daturamycin A and B ( and ), and one new -terphenyl daturamycin C (), along with three known congeners (-), were isolated from a rhizosphere soil-derived sp. KIB-H1544. The structures of new compounds were elucidated via a joint use of spectroscopic analyses and single-crystal X-ray diffractions. Compounds and belong to a rare class of tricyclic 6/5/6 diarylcyclopentenones, and compounds - possess a C-18 tricyclic aromatic skeleton. The biosynthetic gene cluster of daturamycins was identified through gene knockout and biochemical characterization experiments and the biosynthetic pathway of daturamycins was proposed.