Dijk Gerwin, Kaszas Attila, Pas Jolien, O'Connor Rodney Philip
Mines Saint-Etienne, Centre CMP, Department of Bioelectronics, Gardanne, 13541 France.
Panaxium SAS, Aix-en-Provence, 13100 France.
Microsyst Nanoeng. 2022 Aug 29;8:90. doi: 10.1038/s41378-022-00434-7. eCollection 2022.
Transparent microelectrode arrays enable simultaneous electrical recording and optical imaging of neuronal networks in the brain. Electrodes made of the conducting polymer poly(3,4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) are transparent; however, device fabrication necessitates specific processes to avoid deterioration of the organic material. Here, we present an innovative fabrication scheme for a neural probe that consists of transparent PEDOT:PSS electrodes and demonstrate its compatibility with 2-photon microscopy. The electrodes show suitable impedance to record local field potentials from the cortex of mice and sufficient transparency to visualize GCaMP6f-expressing neurons underneath the PEDOT:PSS features. The results validate the performance of the neural probe, which paves the way to study the complex dynamics of in vivo neuronal activity with both a high spatial and temporal resolution to better understand the brain.
透明微电极阵列能够同时对大脑中的神经网络进行电记录和光学成像。由掺杂有聚苯乙烯磺酸盐的导电聚合物聚（3,4-乙撑二氧噻吩）（PEDOT:PSS）制成的电极是透明的；然而，器件制造需要特定的工艺以避免有机材料的劣化。在此，我们展示了一种由透明PEDOT:PSS电极组成的神经探针的创新制造方案，并证明了其与双光子显微镜的兼容性。这些电极显示出适合记录小鼠皮层局部场电位的阻抗，以及足够的透明度以可视化PEDOT:PSS特征下方表达GCaMP6f的神经元。结果验证了神经探针的性能，这为以高空间和时间分辨率研究体内神经元活动的复杂动力学以更好地理解大脑铺平了道路。
