Chen Peifang, Liu Shuang, Yin Zhao, Liang Pengjie, Wang Chunhua, Zhu Hanyue, Liu Yang, Ou Shiyi, Li Guoqiang
Department of Food Science, Foshan University, Foshan, China.
Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China.
Front Nutr. 2022 Aug 16;9:976400. doi: 10.3389/fnut.2022.976400. eCollection 2022.
Acrolein (ACR), an α, β-unsaturated aldehyde, is a toxic compound formed during food processing, and the use of phenolics derived from dietary materials to scavenge ACR is a hot spot. In this study, rutin, a polyphenol widely present in various dietary materials, was used to investigate its capacity to scavenge ACR. It was shown that more than 98% of ACR was eliminated under the conditions of reaction time of 2 h, temperature of 80 °C, and molar ratio of rutin/ACR of 2/1. Further structural characterization of the formed adduct revealed that the adduct of rutin to ACR to form a cyclic hemiacetal compound (RAC) was the main scavenging mechanism. Besides, the stability of RAC during simulated digestion was evaluated, which showed that more than 83.61% of RAC was remained. Furthermore, the cytotoxicity of RAC against Caco-2 and GES-1 cells was significantly reduced compared with ACR, where the IC values of ACR were both below 20 μM while that of RAC were both above 140 μM. And the improvement of the loss of mitochondrial membrane potential (MMP) by RAC might be one of the detoxification pathways. The present study indicated that rutin was one of the potential ACR scavengers among natural polyphenols.
丙烯醛（ACR）是一种α，β-不饱和醛，是食品加工过程中形成的有毒化合物，利用膳食材料中的酚类物质清除ACR是一个研究热点。在本研究中，芦丁作为一种广泛存在于各种膳食材料中的多酚，被用于研究其清除ACR的能力。结果表明，在反应时间为2小时、温度为80℃、芦丁/ACR摩尔比为2/1的条件下，超过98%的ACR被清除。对形成的加合物进行的进一步结构表征表明，芦丁与ACR形成环状半缩醛化合物（RAC）的加合物是主要的清除机制。此外，评估了RAC在模拟消化过程中的稳定性，结果表明超过83.61%的RAC得以保留。此外，与ACR相比，RAC对Caco-2和GES-1细胞的细胞毒性显著降低，其中ACR的IC值均低于20μM，而RAC的IC值均高于140μM。RAC对线粒体膜电位（MMP）损失的改善可能是解毒途径之一。本研究表明，芦丁是天然多酚中潜在的ACR清除剂之一。
