Doctoral School of Exact and Natural Sciences, University of Lodz, 90-236 Lodz, Poland.
Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
Molecules. 2024 May 27;29(11):2519. doi: 10.3390/molecules29112519.
High concentrations of acrolein (2-propenal) are found in polluted air and cigarette smoke, and may also be generated endogenously. Acrolein is also associated with the induction and progression of many diseases. The high reactivity of acrolein towards the thiol and amino groups of amino acids may cause damage to cell proteins. Acrolein may be responsible for the induction of oxidative stress in cells. We hypothesized that acrolein may contribute to the protein damage in erythrocytes, leading to the disruption of the structure of cell membranes. The lipid membrane fluidity, membrane cytoskeleton, and osmotic fragility were measured for erythrocytes incubated with acrolein for 24 h. The levels of thiol, amino, and carbonyl groups were determined in cell membrane and cytosol proteins. The level of non-enzymatic antioxidant potential (NEAC) and TBARS was also measured. The obtained research results showed that the exposure of erythrocytes to acrolein causes changes in the cell membrane and cytosol proteins. Acrolein stiffens the cell membrane of erythrocytes and increases their osmotic sensitivity. Moreover, it has been shown that erythrocytes treated with acrolein significantly reduce the non-enzymatic antioxidant potential of the cytosol compared to the control.
丙烯醛（2-丙烯醛）浓度高存在于污染的空气和香烟烟雾中，也可能内源性生成。丙烯醛也与许多疾病的诱导和进展有关。丙烯醛与氨基酸的巯基和氨基的高反应性可能导致细胞蛋白损伤。丙烯醛可能是细胞氧化应激的诱导因素。我们假设丙烯醛可能导致红细胞中的蛋白质损伤，从而破坏细胞膜的结构。用丙烯醛孵育 24 小时后，测量红细胞的脂质膜流动性、膜细胞骨架和渗透脆性。测定细胞膜和胞浆蛋白中的巯基、氨基和羰基水平。还测量了非酶抗氧化潜力（NEAC）和 TBARS 的水平。研究结果表明，红细胞暴露于丙烯醛会引起细胞膜和胞浆蛋白的变化。丙烯醛使红细胞的细胞膜变硬，增加其渗透敏感性。此外，已经表明，与对照相比，用丙烯醛处理的红细胞显著降低了胞浆中非酶抗氧化潜力。
