鉴定与 SARS-CoV-2 蛋白相互作用的凝血相关蛋白及其遗传变异体与宿主-病毒蛋白相互作用的方案。
Protocol to identify host-viral protein interactions between coagulation-related proteins and their genetic variants with SARS-CoV-2 proteins.
机构信息
Center for Biologics Evaluation and Research, Office of Tissues and Advanced Therapies, Division of Plasma Protein Therapeutics, Food and Drug Administration, Silver Spring, MD, USA.
Center for Biologics Evaluation and Research, Office of Tissues and Advanced Therapies, Division of Plasma Protein Therapeutics, Food and Drug Administration, Silver Spring, MD, USA.
出版信息
STAR Protoc. 2022 Aug 3;3(3):101648. doi: 10.1016/j.xpro.2022.101648. eCollection 2022 Sep 16.
Here, we describe a bioinformatics pipeline that evaluates the interactions between coagulation-related proteins and genetic variants with SARS-CoV-2 proteins. This pipeline searches for host proteins that may bind to viral protein and identifies and scores the protein genetic variants to predict the disease pathogenesis in specific subpopulations. Additionally, it is able to find structurally similar motifs and identify potential binding sites within the host-viral protein complexes to unveil viral impact on regulated biological processes and/or host-protein impact on viral invasion or reproduction. For complete details on the use and execution of this protocol, please refer to Holcomb et al. (2021).
在这里，我们描述了一个生物信息学管道，用于评估与凝血相关的蛋白质与 SARS-CoV-2 蛋白质之间的相互作用。该管道搜索可能与病毒蛋白结合的宿主蛋白，并识别和评分蛋白质遗传变异体，以预测特定亚群中的疾病发病机制。此外，它还能够找到结构相似的基序，并确定宿主-病毒蛋白复合物中的潜在结合位点，以揭示病毒对调控生物过程的影响和/或宿主蛋白对病毒入侵或复制的影响。有关此方案的使用和执行的详细信息，请参阅 Holcomb 等人。（2021 年）。
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